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Home > Curated Information Page > PubMed Id: 15143153
Gao Z, et al. (2004) Inhibition of insulin sensitivity by free fatty acids requires activation of multiple serine kinases in 3T3-L1 adipocytes. Mol Endocrinol 18, 2024-34 15143153
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S307-p - IRS1 (human)
Modsite: TRRSRTEsITATSPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
INSR (human) Disrupts co-immunoprecipitation

T308-p - Akt1 (mouse)
Modsite: KDGATMKtFCGTPEY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
linoleic_acid no change compared to control
linoleic_acid insulin inhibit treatment-induced increase

S180-p - IKKA (mouse)
Modsite: DQGSLCTsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

S307-p - IRS1 (mouse)
Modsite: TESITATsPASMVGG SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKA (mouse) pharmacological inhibitor of upstream enzyme
KINASE JNK1 (mouse) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
calphostin_C phorbol_ester inhibit treatment-induced increase
SP600125 linoleic_acid inhibit treatment-induced increase
linoleic_acid increase
calphostin_C linoleic_acid inhibit treatment-induced increase
15d-PGJ2, linoleic_acid linoleic_acid inhibit treatment-induced increase

Y628-p - IRS1 (mouse)
Modsite: NRKGNGDyMPMSPKS SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): Y632‑p, IRS1 (mouse): Y628‑p, IRS1 (rat): Y628‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
linoleic_acid insulin inhibit treatment-induced increase

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPYVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

Y185-p - JNK2 (mouse)
Modsite: TNFMMTPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

T538-p - PKCT (mouse)
Modsite: LGDAKTNtFCGTPDY SwissProt Entrez-Gene
Orthologous residues
PKCT (human): T538‑p, PKCT (mouse): T538‑p, PKCT (rat): T538‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

T410-p - PKCZ (mouse)
Modsite: GPGDTTStFCGTPNY SwissProt Entrez-Gene
Orthologous residues
PKCZ (human): T410‑p, PKCZ iso2 (human): T227‑p, PKCZ iso3 (human): T306‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

S744-p - PRKD1 (mouse)
Modsite: ARIIGEKsFRRSVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase

S748-p - PRKD1 (mouse)
Modsite: GEKSFRRsVVGTPAY SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
linoleic_acid increase