Curated Information
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Home > Curated Information Page > PubMed Id: 15319431
Gentile MT, et al. (2004) Mechanisms of soluble beta-amyloid impairment of endothelial function. J Biol Chem 279, 48135-42 15319431
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S117-p - eNOS (rat)
Modsite: SRPTQGPsPTEQLLG SwissProt Entrez-Gene
Orthologous residues
eNOS (human): P118‑p, eNOS (mouse): S117‑p, eNOS (rat): S117‑p, eNOS (rabbit): P124‑p, eNOS (pig): P120‑p, eNOS (cow): P120‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  endothelial-aorta
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
beta-amyloid_40 increase
ACh beta-amyloid_40 no effect upon treatment-induced increase
Go_6976 beta-amyloid_40 inhibit treatment-induced increase
ACh no change compared to control
Go_6976 no change compared to control

T494-p - eNOS (rat)
Modsite: TGITRKKtFKEVANA SwissProt Entrez-Gene
Orthologous residues
eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  endothelial-aorta
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
beta-amyloid_40 increase
ACh beta-amyloid_40 no effect upon treatment-induced increase
Go_6976 beta-amyloid_40 inhibit treatment-induced increase
ACh no change compared to control
Go_6976 no change compared to control

S1176-p - eNOS (rat)
Modsite: TSRIRtQsFsLQERQ SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  endothelial-aorta
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ACh increase
beta-amyloid_40 ACh inhibit treatment-induced increase
Go_6976 ACh no effect upon treatment-induced increase
beta-amyloid_40 no change compared to control
Go_6976 no change compared to control

S661-p - PKCB (rat)
Modsite: QNEFAGFsYTNPEFV SwissProt Entrez-Gene
Orthologous residues
PKCB (human): S661‑p, PKCB iso2 (human): , PKCB (mouse): S661‑p, PKCB iso2 (mouse): , PKCB (rat): S661‑p, PKCB iso2 (rat): , PKCB (cow):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  endothelial-aorta
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
beta-amyloid_40 increase