Drew BG, et al. (2004) High-density lipoprotein and apolipoprotein AI increase endothelial NO synthase activity by protein association and multisite phosphorylation. Proc Natl Acad Sci U S A 101, 6999-7004 15107497

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S80-p - ACC1 (human) ▲

Modsite: LHIRssMsGLHLVkQ SwissProt Entrez-Gene Orthologous residues ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes acadesine no change compared to control DNP increase sorbitol increase

T183-p - AMPKA1 (human) ▲

Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene Orthologous residues AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I increase

S116-p - eNOS (cow) ▲

Modsite: RKLQTRPsPGPPPAE SwissProt Entrez-Gene Orthologous residues eNOS (human): S114‑p, eNOS (mouse): T113‑p, eNOS (rat): T113‑p, eNOS (rabbit): T120‑p, eNOS (pig): S116‑p, eNOS (cow): S116‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I increase HDL increase LDL no change compared to control

T497-p - eNOS (cow) ▲

Modsite: AGITRKktFKEVANA SwissProt Entrez-Gene Orthologous residues eNOS (human): T495‑p, eNOS (mouse): T494‑p, eNOS (rat): T494‑p, eNOS (rabbit): T501‑p, eNOS (pig): T497‑p, eNOS (cow): T497‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I decrease HDL decrease LDL no change compared to control

S617-p - eNOS (cow) ▲

Modsite: SYKIRFNsVSCSDPL SwissProt Entrez-Gene Orthologous residues eNOS (human): S615‑p, eNOS (mouse): S614‑p, eNOS (rat): S614‑p, eNOS (rabbit): S621‑p, eNOS (pig): S617‑p, eNOS (cow): S617‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I no change compared to control HDL no change compared to control LDL increase

S635-p - eNOS (cow) ▲

Modsite: WRRKRKEsSNTDSAG SwissProt Entrez-Gene Orthologous residues eNOS (human): S633‑p, eNOS (mouse): S632‑p, eNOS (rat): S632‑p, eNOS (rabbit): S639‑p, eNOS (pig): S635‑p, eNOS (cow): S635‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I increase HDL increase LDL increase

S1179-p - eNOS (cow) ▲

Modsite: TSRIRtQsFsLQERH SwissProt Entrez-Gene Orthologous residues eNOS (human): S1177‑p, eNOS (mouse): S1176‑p, eNOS (rat): S1176‑p, eNOS (rabbit): S1183‑p, eNOS (pig): S1179‑p, eNOS (cow): S1179‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  endothelial-aorta Cellular systems studied:  primary cultured cells Species studied:  cow Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes apoA-I increase HDL increase