Curated Information
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Home > Curated Information Page > PubMed Id: 29101251
Wang C, et al. (2017) The methyltransferase NSD3 promotes antiviral innate immunity via direct lysine methylation of IRF3. J Exp Med 214, 3597-3610 29101251
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K366-m1 - IRF3 (human)
Modsite: TKRLVMVkVVPtCLR SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): K366‑m1, IRF3 (mouse): K359‑m1, IRF3 (rat): K361‑m1
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-peritoneum, MEF (fibroblast), RAW 264.7 (macrophage)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE WHSC1L1 (human) activation of upstream enzyme, co-immunoprecipitation, transfection of inactive enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
virus infection increase HSV and VSV infection
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PPP1CC (human) Disrupts co-immunoprecipitation, microscopy-colocalization
Comments:  induces antiviral innate immune response