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Home > Curated Information Page > PubMed Id: 15634336
Hekerman P, et al. (2005) Pleiotropy of leptin receptor signalling is defined by distinct roles of the intracellular tyrosines. FEBS J 272, 109-19 15634336
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y985-p - LEPR (mouse)
Modsite: QRQPSVKyATLVSND SwissProt Entrez-Gene
Orthologous residues
LEPR (human): Y986‑p, LEPR iso2 (human): , LEPR (mouse): Y985‑p, LEPR iso6 (mouse): Y985‑p, LEPR (rat): Y985‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  hamster
Downstream Regulation
Effect of modification (function):  activity, induced

Y1077-p - LEPR (mouse)
Modsite: HREKSVCyLGVTSVN SwissProt Entrez-Gene
Orthologous residues
LEPR (human): Y1079‑p, LEPR iso2 (human): , LEPR (mouse): Y1077‑p, LEPR iso6 (mouse): Y1077‑p, LEPR (rat): Y1077‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  hamster
Downstream Regulation
Effect of modification (function):  activity, induced

Y1138-p - LEPR (mouse)
Modsite: SGENFVPyMPQFQTC SwissProt Entrez-Gene
Orthologous residues
LEPR (human): Y1141‑p, LEPR iso2 (human): , LEPR (mouse): Y1138‑p, LEPR iso6 (mouse): Y1138‑p, LEPR (rat): Y1138‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic)
Cellular systems studied:  cell lines
Species studied:  hamster
Downstream Regulation
Effect of modification (function):  activity, induced

T183-p - AMPKA1 (rat)
Modsite: SDGEFLRtsCGSPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin no change compared to control

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y985 was necessary and sufficient for leptin-induced activation of ERK1/2.

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y985 was necessary and sufficient for leptin-induced activation of ERK1/2.

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y985 was necessary and sufficient for leptin-induced activation of ERK1/2.

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y985 was necessary and sufficient for leptin-induced activation of ERK1/2.

Y701-p - STAT1 (rat)
Modsite: DDPKRTGyIKTELIS SwissProt Entrez-Gene
Orthologous residues
STAT1 (human): Y701‑p, STAT1 iso2 (human): Y701‑p, STAT1 (mouse): Y701‑p, STAT1 (rat): Y701‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y1138 was necessary for leptin-induced activation of STAT1, STAT3, and STAT5.
IL-6 increase
GH increase

Y705-p - STAT3 (rat)
Modsite: DPGSAAPyLKTKFIC SwissProt Entrez-Gene
Orthologous residues
STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase In mutagenesis studies, LEPR Y1138 was necessary for leptin-induced activation of STAT1, STAT3, and STAT5.
IL-6 increase

Y694-p - STAT5A (rat)
Modsite: LAKAVDGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5A (human): Y694‑p, STAT5A (mouse): Y694‑p, STAT5A iso2 (mouse): , STAT5A (rat): Y694‑p, STAT5A (fruit fly): Y711‑p, STAT5A (sheep): Y694‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
leptin increase

Y699-p - STAT5B (rat)
Modsite: TAKAADGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin LEPR (mouse) increase In mutagenesis studies, LEPR Y1077 mediated the leptin-induced activaton of STAT5, while Y1138 was involved in activation of STAT1, STAT3, and STAT5.
GH increase

Y641-p - STAT6 (rat)
Modsite: MGKDGRGyVSTTIKM SwissProt Entrez-Gene
Orthologous residues
STAT6 (human): Y641‑p, STAT6 iso2 (human): Y467‑p, STAT6 (mouse): Y641‑p, STAT6 (rat): Y641‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  HIT-T15 (pancreatic), RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  hamster, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
leptin increase