Curated Information
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Home > Curated Information Page > PubMed Id: 12167711
Blattner C, Hay T, Meek DW, Lane DP (2002) Hypophosphorylation of Mdm2 augments p53 stability. Mol Cell Biol 22, 6170-82 12167711
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S240-p - MDM2 (human)
Modsite: GDWLDQDsVsDQFsV SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S240‑p, MDM2 (mouse): S238‑p, MDM2 (rat): S208‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S242-p - MDM2 (human)
Modsite: WLDQDsVsDQFsVEF SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S242‑p, MDM2 (mouse): S240‑p, MDM2 (rat): S210‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S246-p - MDM2 (human)
Modsite: DsVsDQFsVEFEVEs SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S246‑p, MDM2 (mouse): S244‑p, MDM2 (rat): S214‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S253-p - MDM2 (human)
Modsite: sVEFEVEsLDsEDYs SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S253‑p, MDM2 (mouse): S251‑p, MDM2 (rat): S221‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S256-p - MDM2 (human)
Modsite: FEVEsLDsEDYsLsE SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S256‑p, MDM2 (mouse): S254‑p, MDM2 (rat): S224‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S260-p - MDM2 (human)
Modsite: sLDsEDYsLsEEGQE SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S260‑p, MDM2 (mouse): S258‑p, MDM2 (rat): S228‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation

S262-p - MDM2 (human)
Modsite: DsEDYsLsEEGQELs SwissProt Entrez-Gene
Orthologous residues
MDM2 (human): S262‑p, MDM2 (mouse): S260‑p, MDM2 (rat): S230‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphopeptide mapping, western blotting
Disease tissue studied:  lung cancer, non-small cell lung cancer
Relevant cell lines - cell types - tissues:  COS (fibroblast), GM1604 (fibroblast), NCI-H1299 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation decrease
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  promotes p53 degradation