Curated Information
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Home > Curated Information Page > PubMed Id: 15611134
Graves PR, Winkfield KM, Haystead TA (2005) Regulation of zipper-interacting protein kinase activity in vitro and in vivo by multisite phosphorylation. J Biol Chem 280, 9363-74 15611134
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T180-p - DAPK3 (human)
Modsite: EFKNIFGtPEFVAPE SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): T180‑p, DAPK3 (mouse): T180‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T225-p - DAPK3 (human)
Modsite: LGETKQEtLTNISAV SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): T225‑p, DAPK3 (mouse): T225‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T265-p - DAPK3 (human)
Modsite: KDPKRRMtIAQSLEH SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): T265‑p, DAPK3 (mouse): T265‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T299-p - DAPK3 (human)
Modsite: PERRRLKtTRLkEyt SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): T299‑p, DAPK3 (mouse): A294‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization

T306-p - DAPK3 (human)
Modsite: tTRLkEytIksHssL SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): T306‑p, DAPK3 (mouse): S301‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)

S311-p - DAPK3 (human)
Modsite: EytIksHssLPPNNS SwissProt Entrez-Gene
Orthologous residues
DAPK3 (human): S311‑p, DAPK3 (mouse): S306‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE DAPK3 (human)