Curated Information
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Home > Curated Information Page > PubMed Id: 18652571
Murugappan S, et al. (2009) Differential regulation of threonine and tyrosine phosphorylations on protein kinase Cdelta by G-protein-mediated pathways in platelets. Biochem J 417, 113-20 18652571
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y313-p - PKCD (human)
Modsite: ssEPVGIyQGFEKkT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), platelet-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  green monkey, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DiC8 no change compared to control
phorbol_ester increase
PAR1-activating_peptide no change compared to control YFLLRNP
DiC8, PAR1-activating_peptide increase YFLLRNP
PAR4-activating_peptide no change compared to control YM-254890
DiC8, PAR4-activating_peptide increase YM-254890
PP2 DiC8, PAR4-activating_peptide inhibit treatment-induced increase
thrombin increase

T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), platelet-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  green monkey, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DiC8 increase
PAR1-activating_peptide DiC8 augment treatment-induced increase YFLLRNP
phorbol_ester increase
PAR1-activating_peptide no change compared to control YFLLRNP
PAR4-activating_peptide no change compared to control
thrombin increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  required for Y313 phosphorylation

Y311-p - PKCD (mouse)
Modsite: TTEsVGIyQGFEKKP SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), platelet-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  green monkey, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DiC8 no change compared to control
PAR4-activating_peptide no change compared to control
DiC8, PAR4-activating_peptide increase
PP2 DiC8, PAR4-activating_peptide inhibit treatment-induced increase

T505-p - PKCD (mouse)
Modsite: FGEGRAstFCGtPDY SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), platelet-blood
Cellular systems studied:  cell lines, primary cells
Species studied:  green monkey, human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DiC8 increase
PAR4-activating_peptide DiC8 no effect upon treatment-induced increase
DiC8, PAR4-activating_peptide increase
PP2 DiC8, PAR4-activating_peptide no effect upon treatment-induced increase