Ikuno Y, Leong FL, Kazlauskas A (2002) PI3K and PLCgamma play a central role in experimental PVR. Invest Ophthalmol Vis Sci 43, 483-9 11818394

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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Y572-p - PDGFRA (human) ▲

Modsite: IsPDGHEyIyVDPMQ SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y572‑p, PDGFRA (mouse): Y572‑p, PDGFRA (rat): Y571‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase

Y574-p - PDGFRA (human) ▲

Modsite: PDGHEyIyVDPMQLP SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y574‑p, PDGFRA (mouse): Y574‑p, PDGFRA (rat): Y573‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase

Y720-p - PDGFRA (human) ▲

Modsite: ADEstRSyVILSFEN SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y720‑p, PDGFRA (mouse): Y720‑p, PDGFRA (rat): Y719‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SHP-2 (mouse) Induces co-immunoprecipitation

Y731-p - PDGFRA (human) ▲

Modsite: SFENNGDyMDMKQAD SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y731‑p, PDGFRA (mouse): Y731‑p, PDGFRA (rat): Y730‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIK3R1 (mouse) Induces co-immunoprecipitation

Y742-p - PDGFRA (human) ▲

Modsite: KQADttQyVPMLERK SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y742‑p, PDGFRA (mouse): Y742‑p, PDGFRA (rat): Y741‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIK3R1 (mouse) Induces co-immunoprecipitation

Y988-p - PDGFRA (human) ▲

Modsite: RVDSDNAyIGVtyKN SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y988‑p, PDGFRA (mouse): Y988‑p, PDGFRA (rat): Y987‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase

Y1018-p - PDGFRA (human) ▲

Modsite: RLSADsGyIIPLPDI SwissProt Entrez-Gene Orthologous residues PDGFRA (human): Y1018‑p, PDGFRA (mouse): Y1018‑p, PDGFRA (rat): Y1017‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  F cells (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PDGF increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PLCG1 (mouse) Induces co-immunoprecipitation