Sidi S, et al. (2008) Chk1 suppresses a caspase-2 apoptotic response to DNA damage that bypasses p53, Bcl-2, and caspase-3. Cell 133, 864-77 18510930

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S216-p - CDC25C (human) ▲

Modsite: sGLyRsPsMPENLNR SwissProt Entrez-Gene Orthologous residues CDC25C (human): S216‑p, CDC25C iso3 (human): S173‑p, CDC25C (mouse): ‑, CDC25C (frog): S287‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ionizing_radiation decrease

Y15-p - CDK1 (human) ▲

Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene Orthologous residues CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Chk1 (human) decrease depletion ionizing_radiation decrease

S317-p - Chk1 (human) ▲

Modsite: ENVkYsssQPEPRTG SwissProt Entrez-Gene Orthologous residues Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Go_6976 increase ionizing_radiation increase Go_6976 ionizing_radiation augment treatment-induced increase

T68-p - Chk2 (human) ▲

Modsite: SsLEtVstQELYsIP SwissProt Entrez-Gene Orthologous residues Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Go_6976 increase ionizing_radiation Go_6976 augment treatment-induced increase

S139-p - H2AX (human) ▲

Modsite: GkkAtQAsQEy____ SwissProt Entrez-Gene Orthologous residues H2AX (human): S139‑p, H2AX (mouse): S139‑p, H2AX (rat): S139‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Go_6976 increase ionizing_radiation increase Go_6976 ionizing_radiation augment treatment-induced increase

S82-p - HSP27 (human) ▲

Modsite: RALsRQLssGVsEIr SwissProt Entrez-Gene Orthologous residues HSP27 (human): S82‑p, HSP27 (mouse): S86‑p, HSP27 (rat): S86‑p, HSP27 (pig): S84‑p, HSP27 (hamster): S90‑p, HSP27 (chicken): S80‑p, HSP27 (dog): S86‑p, HSP27 (cow): S78‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  embryo, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human Comments:  zebrafish embryo Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes ionizing_radiation increase SB203580 ionizing_radiation inhibit treatment-induced increase