Curated Information
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Home > Curated Information Page > PubMed Id: 18587398
Butler B, Kastendieck DH, Cooper JA (2008) Differently phosphorylated forms of the cortactin homolog HS1 mediate distinct functions in natural killer cells. Nat Immunol 9, 887-97 18587398
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y378-p - HS1 (human)
Modsite: EPEPENDyEDVEEMD SwissProt Entrez-Gene
Orthologous residues
HS1 (human): Y378‑p, HS1 (mouse): Y388‑p, HS1 (rat): Y378‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human

Y397-p - HS1 (human)
Modsite: EDEPEGDyEEVLEPE SwissProt Entrez-Gene
Orthologous residues
HS1 (human): Y397‑p, HS1 (mouse): Y405‑p, HS1 (rat): Y395‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell adhesion, altered, cytoskeletal reorganization

Y397-p - LYN (human)
Modsite: RVIEDNEytAREGAk SwissProt Entrez-Gene
Orthologous residues
LYN (human): Y397‑p, LYN iso2 (human): Y376‑p, LYN (mouse): Y397‑p, LYN iso2 (mouse): Y376‑p, LYN (rat): Y397‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin, phorbol_ester HS1 (human) increase
siRNA fibronectin, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA
phorbol_ester, ICAM-1 HS1 (human) increase
siRNA ICAM-1, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA

Y467-p - PIK3R1 (human)
Modsite: SREyDRLyEEytRTS SwissProt Entrez-Gene
Orthologous residues
PIK3R1 (human): Y467‑p, PIK3R1 iso2 (human): Y197‑p, PIK3R1 iso4 (human): Y467‑p, PIK3R1 (mouse): Y467‑p, PIK3R1 (rat): Y467‑p, PIK3R1 (cow): Y467‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin, phorbol_ester HS1 (human) increase
siRNA fibronectin, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA
phorbol_ester, ICAM-1 HS1 (human) increase
siRNA ICAM-1, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin, phorbol_ester HS1 (human) increase
siRNA fibronectin, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA
phorbol_ester, ICAM-1 HS1 (human) increase
siRNA ICAM-1, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA

Y174-p - VAV1 (human)
Modsite: EAEGDEIyEDLMRsE SwissProt Entrez-Gene
Orthologous residues
VAV1 (human): Y174‑p, VAV1 iso2 (human): Y174‑p, VAV1 (mouse): Y174‑p, VAV1 (rat): Y174‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  natural killer cell-blood
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin, phorbol_ester HS1 (human) increase
siRNA fibronectin, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA
phorbol_ester, ICAM-1 HS1 (human) increase
siRNA ICAM-1, phorbol_ester HS1 (human) inhibit treatment-induced increase HS1 siRNA