Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18378685
Ozgen N, et al. (2008) Protein kinase D links Gq-coupled receptors to cAMP response element-binding protein (CREB)-Ser133 phosphorylation in the heart. J Biol Chem 283, 17009-19 18378685
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S133-p - CREB (rat)
Modsite: EILsRRPsYRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
phorbol_ester increase
thrombin increase
EGF increase
U0126 phorbol_ester, thrombin no effect upon treatment-induced increase
U0126 EGF inhibit treatment-induced increase
AG1478 phorbol_ester, thrombin no effect upon treatment-induced increase
AG1478 EGF inhibit treatment-induced increase
GF109203X norepinephrine, phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 norepinephrine, phorbol_ester, thrombin inhibit treatment-induced increase
H2O2 increase
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
U0126 norepinephrine no effect upon treatment-induced increase
Pasteurella_multocida_toxin increase
Go_6976 inhibit treatment-induced increase
U0126 Pasteurella_multocida_toxin no effect upon treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
norepinephrine increase
prazosin norepinephrine inhibit treatment-induced increase
propranolol norepinephrine no effect upon treatment-induced increase
isoproterenol no change compared to control
Pasteurella_multocida_toxin increase
Go_6976 Pasteurella_multocida_toxin no effect upon treatment-induced increase
U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
PRKD1 (rat) no change compared to control PKD S744E/748E mutant over expression
phorbol_ester PRKD1 (rat) increase
GF109203X phorbol_ester no effect upon treatment-induced increase
Go_6976 phorbol_ester inhibit treatment-induced increase
PRKD1 (rat) no change compared to control PKD S744E/748E mutant over expression
Pasteurella_multocida_toxin PRKD1 (rat) increase
PKCD (rat) increase
Pasteurella_multocida_toxin PKCD (rat) augment treatment-induced increase
PKCE (rat) no change compared to control
Pasteurella_multocida_toxin PKCE (rat) increase
PKCA (rat) no change compared to control
Pasteurella_multocida_toxin PKCA (rat) increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  transcription, altered
Comments:  Pasteurella multocida toxin induced nuclear localization and CREB transactivation

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
EGF augment treatment-induced decrease
phorbol_ester increase
U0126 EGF, phorbol_ester, thrombin inhibit treatment-induced increase
AG1478 EGF, thrombin inhibit treatment-induced increase
AG1478 phorbol_ester no effect upon treatment-induced increase
thrombin increase
phorbol_ester increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 phorbol_ester, thrombin no effect upon treatment-induced increase
H2O2 increase
augment treatment-induced decrease
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
GF109203X norepinephrine inhibit treatment-induced increase
Go_6976 norepinephrine no effect upon treatment-induced increase
U0126 no effect upon treatment-induced increase
Pasteurella_multocida_toxin increase
Go_6976 Pasteurella_multocida_toxin no effect upon treatment-induced increase
U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
EGF augment treatment-induced decrease
phorbol_ester increase
U0126 EGF, phorbol_ester, thrombin inhibit treatment-induced increase
AG1478 EGF, thrombin inhibit treatment-induced increase
AG1478 phorbol_ester no effect upon treatment-induced increase
thrombin increase
phorbol_ester increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 phorbol_ester, thrombin no effect upon treatment-induced increase
H2O2 increase
augment treatment-induced decrease
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
GF109203X norepinephrine inhibit treatment-induced increase
Go_6976 norepinephrine no effect upon treatment-induced increase
U0126 no effect upon treatment-induced increase
Pasteurella_multocida_toxin increase
Go_6976 Pasteurella_multocida_toxin no effect upon treatment-induced increase
U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
EGF augment treatment-induced decrease
phorbol_ester increase
U0126 EGF, phorbol_ester, thrombin inhibit treatment-induced increase
AG1478 EGF, thrombin inhibit treatment-induced increase
AG1478 phorbol_ester no effect upon treatment-induced increase
thrombin increase
phorbol_ester increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 phorbol_ester, thrombin no effect upon treatment-induced increase
H2O2 increase
augment treatment-induced decrease
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
GF109203X norepinephrine inhibit treatment-induced increase
Go_6976 norepinephrine no effect upon treatment-induced increase
U0126 no effect upon treatment-induced increase
Pasteurella_multocida_toxin increase
Go_6976 Pasteurella_multocida_toxin no effect upon treatment-induced increase
U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
EGF augment treatment-induced decrease
phorbol_ester increase
U0126 EGF, phorbol_ester, thrombin inhibit treatment-induced increase
AG1478 EGF, thrombin inhibit treatment-induced increase
AG1478 phorbol_ester no effect upon treatment-induced increase
thrombin increase
phorbol_ester increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 phorbol_ester, thrombin no effect upon treatment-induced increase
H2O2 increase
augment treatment-induced decrease
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
GF109203X norepinephrine inhibit treatment-induced increase
Go_6976 norepinephrine no effect upon treatment-induced increase
U0126 no effect upon treatment-induced increase
Pasteurella_multocida_toxin increase
Go_6976 Pasteurella_multocida_toxin no effect upon treatment-induced increase
U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase
Go_6976, U0126 Pasteurella_multocida_toxin inhibit treatment-induced increase

T573-p - p90RSK (rat)
Modsite: AENGLLMtPCYTANF SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T573‑p, p90RSK iso2 (human): T582‑p, p90RSK (mouse): T562‑p, p90RSK iso3 (mouse): , p90RSK (rat): T573‑p, p90RSK (chicken): T590‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
EGF augment treatment-induced decrease
phorbol_ester increase
U0126 EGF, phorbol_ester, thrombin inhibit treatment-induced increase
AG1478 EGF, thrombin inhibit treatment-induced increase
AG1478 phorbol_ester no effect upon treatment-induced increase
PRKD1 (rat) no change compared to control PKD S744E/748E mutant over expression
Pasteurella_multocida_toxin PRKD1 (rat) increase
PKCD (rat) increase
Pasteurella_multocida_toxin PKCD (rat) augment treatment-induced increase
PKCE (rat) no change compared to control
Pasteurella_multocida_toxin PKCE (rat) increase
PKCA (rat) no change compared to control
Pasteurella_multocida_toxin PKCA (rat) increase

T505-p - PKCD (rat)
Modsite: FGENRAstFCGTPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
phorbol_ester increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
Go_6976 phorbol_ester, thrombin no effect upon treatment-induced increase
norepinephrine increase
prazosin norepinephrine inhibit treatment-induced increase
propranolol norepinephrine no effect upon treatment-induced increase
isoproterenol no change compared to control
H2O2 increase
augment treatment-induced decrease
GF109203X H2O2 decrease
Go_6976 H2O2 decrease
norepinephrine increase
GF109203X norepinephrine inhibit treatment-induced increase
Go_6976 norepinephrine no effect upon treatment-induced increase
U0126 no effect upon treatment-induced increase

S744-p - PRKD1 (rat)
Modsite: ARIIGEKsFRRsVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
phorbol_ester increase
AG1478 phorbol_ester, thrombin no effect upon treatment-induced increase
U0126 phorbol_ester, thrombin no effect upon treatment-induced increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
norepinephrine increase
prazosin norepinephrine inhibit treatment-induced increase
propranolol norepinephrine no effect upon treatment-induced increase
isoproterenol no change compared to control

S748-p - PRKD1 (rat)
Modsite: GEKsFRRsVVGTPAY SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
phorbol_ester increase
AG1478 phorbol_ester, thrombin no effect upon treatment-induced increase
U0126 phorbol_ester, thrombin no effect upon treatment-induced increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
norepinephrine increase
prazosin norepinephrine inhibit treatment-induced increase
propranolol norepinephrine no effect upon treatment-induced increase
isoproterenol no change compared to control

S916-p - PRKD1 (rat)
Modsite: KALSERVsIL_____ SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S910‑p, PRKD1 (mouse): S916‑p, PRKD1 (rat): S916‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-heart, myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
thrombin increase
Pasteurella_multocida_toxin increase
thrombin increase
phorbol_ester increase
AG1478 phorbol_ester, thrombin no effect upon treatment-induced increase
U0126 phorbol_ester, thrombin no effect upon treatment-induced increase
GF109203X phorbol_ester, thrombin inhibit treatment-induced increase
norepinephrine increase
prazosin norepinephrine inhibit treatment-induced increase
propranolol norepinephrine no effect upon treatment-induced increase
isoproterenol no change compared to control
PRKD1 (rat) no change compared to control PKD S744E/748E mutant over expression
phorbol_ester PRKD1 (rat) increase
GF109203X phorbol_ester no effect upon treatment-induced increase
Go_6976 phorbol_ester inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  transcription, altered
Comments:  Pasteurella multocida toxin induced nuclear localization and CREB transactivation