Curated Information
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Home > Curated Information Page > PubMed Id: 15364936
Ye X, et al. (2004) Recognition of phosphodegron motifs in human cyclin E by the SCF(Fbw7) ubiquitin ligase. J Biol Chem 279, 50110-9 15364936
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T77-p - CCNE1 (human)
Modsite: DPCsLIPtPDKEDDD SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): T77‑p, CCNE1 (mouse): T74‑p, CCNE1 (rat): T77‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXW7 (human) Induces

S103-p - CCNE1 (human)
Modsite: IIAPSRGsPLPVLSW SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): S103‑p, CCNE1 (mouse): S101‑p, CCNE1 (rat): S104‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S387-p - CCNE1 (human)
Modsite: LsEQNRAsPLPSGLL SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): S387‑p, CCNE1 (mouse): S385‑p, CCNE1 (rat): S388‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T395-p - CCNE1 (human)
Modsite: PLPSGLLtPPQsGKK SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): T395‑p, CCNE1 (mouse): T393‑p, CCNE1 (rat): T396‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, peptide sequencing, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXW7 (human) Induces

S399-p - CCNE1 (human)
Modsite: GLLtPPQsGKKQSSG SwissProt Entrez-Gene
Orthologous residues
CCNE1 (human): S399‑p, CCNE1 (mouse): S397‑p, CCNE1 (rat): S400‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, peptide sequencing
Relevant cell lines - cell types - tissues:  HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation