Yi P, et al. (2008) Atypical protein kinase C regulates dual pathways for degradation of the oncogenic coactivator SRC-3/AIB1. Mol Cell 29, 465-76 18313384

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S1033-p - SRC-3 (human) ▲

Modsite: NRPLLRNsLDDLVGP SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S1033‑p, SRC‑3 iso2 (human): S1018‑p, SRC‑3 (mouse): S1040‑p, SRC‑3 (rat): S722‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

S1042-p - SRC-3 (human) ▲

Modsite: DDLVGPPsNLEGQsD SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S1042‑p, SRC‑3 iso2 (human): S1027‑p, SRC‑3 (mouse): S1049‑p, SRC‑3 (rat): S731‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

S1048-p - SRC-3 (human) ▲

Modsite: PsNLEGQsDERALLD SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S1048‑p, SRC‑3 iso2 (human): S1033‑p, SRC‑3 (mouse): S1055‑p, SRC‑3 (rat): S737‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

T1059-p - SRC-3 (human) ▲

Modsite: ALLDQLHtLLsNtDA SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): T1059‑p, SRC‑3 iso2 (human): T1044‑p, SRC‑3 (mouse): T1066‑p, SRC‑3 (rat): T748‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

S1062-p - SRC-3 (human) ▲

Modsite: DQLHtLLsNtDAtGL SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): S1062‑p, SRC‑3 iso2 (human): S1047‑p, SRC‑3 (mouse): S1069‑p, SRC‑3 (rat): S751‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

T1064-p - SRC-3 (human) ▲

Modsite: LHtLLsNtDAtGLEE SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): T1064‑p, SRC‑3 iso2 (human): T1049‑p, SRC‑3 (mouse): T1071‑p, SRC‑3 (rat): T753‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

T1067-p - SRC-3 (human) ▲

Modsite: LLsNtDAtGLEEIDR SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): T1067‑p, SRC‑3 iso2 (human): T1052‑p, SRC‑3 (mouse): T1074‑p, SRC‑3 (rat): T756‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues

T1114-p - SRC-3 (human) ▲

Modsite: KAGLYGQtYPAQGPP SwissProt Entrez-Gene Orthologous residues SRC‑3 (human): T1114‑p, SRC‑3 iso2 (human): T1099‑p, SRC‑3 (mouse): T1121‑p, SRC‑3 (rat): T803‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKCZ (human) co-immunoprecipitation, transfection of wild-type enzyme Comments:  some subset of these 8 residues Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PSMA3 (human) Disrupts pull-down assay Comments:  some subset of these 8 residues