Curated Information
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Home > Curated Information Page > PubMed Id: 18299321
Kim SI, Kwak JH, Wang L, Choi ME (2008) Protein phosphatase 2A is a negative regulator of transforming growth factor-beta1-induced TAK1 activation in mesangial cells. J Biol Chem 283, 10753-63 18299321
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T187-p - TAK1 (human)
Modsite: CDIQtHMtNNKGsAA SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T187‑p, TAK1 (mouse): T187‑p, TAK1 iso3 (mouse): T187‑p, TAK1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  mesangial
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Comments:  in the presence of TAB1
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (mouse) transfection of inactive enzyme, siRNA inhibition of enzyme
KINASE TAK1 (mouse) pharmacological activator of upstream enzyme, transfection of dominant-negative enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase
TGF-beta increase
okadaic_acid TGF-beta augment treatment-induced increase
siRNA TGF-beta PPP2CA (mouse) augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S439-p - TAK1 (human)
Modsite: NGQPRRRsIQDLtVT SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): S439‑p, TAK1 (mouse): S412‑p, TAK1 iso3 (mouse): S439‑p, TAK1 (rat): S439‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  mesangial
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Comments:  in the presence of TAB1
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (mouse) transfection of inactive enzyme, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid no change compared to control