Curated Information
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Home > Curated Information Page > PubMed Id: 18285345
Waraich RS, et al. (2008) Phosphorylation of Ser357 of rat insulin receptor substrate-1 mediates adverse effects of protein kinase C-delta on insulin action in skeletal muscle cells. J Biol Chem 283, 11226-33 18285345
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin PKCD (mouse) increase PKCD WT
phorbol_ester insulin PKCD (mouse) PKCD (mouse) decrease WT PKCD
insulin PKCD (mouse) increase KN PKCD
phorbol_ester insulin PKCD (mouse) PKCD (mouse) inhibit treatment-induced increase KN PKCD
insulin PKCD (mouse) increase WT PKCD IRS1357/358A mutant
phorbol_ester insulin PKCD (mouse) inhibit treatment-induced increase WT PKCD IRS1357/358A mutant

S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
insulin IRS1 (rat) augment treatment-induced increase IRS1 mutant S357/358A

S357-p - IRS1 (rat)
Modsite: HAHRHRGssRLHPPL SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S362‑p, IRS1 (mouse): S357‑p, IRS1 (rat): S357‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCD (rat) transfection of dominant-negative enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PKCD (rat) Induces co-immunoprecipitation
Comments:  leads to reduced phosphorylation of Akt

S358-p - IRS1 (rat)
Modsite: AHRHRGssRLHPPLN SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): A363‑p, IRS1 (mouse): S358‑p, IRS1 (rat): S358‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse