Curated Information
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Home > Curated Information Page > PubMed Id: 18252715
Kim YB, et al. (2008) Cell adhesion-dependent cofilin serine 3 phosphorylation by the integrin-linked kinase.c-Src complex. J Biol Chem 283, 10089-96 18252715
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion ILK (human) increase ILK adenovirus
fibronectin increase
LY294002 fibronectin no effect upon treatment-induced increase
PP2 fibronectin inhibit treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
p18_peptide fibronectin no effect upon treatment-induced increase
Y27632 fibronectin inhibit treatment-induced increase

S3-p - Cofilin-1 (rat)
Modsite: _____MAsGVAVsDG SwissProt Entrez-Gene
Orthologous residues
Cofilin‑1 (human): S3‑p, Cofilin‑1 (mouse): S3‑p, Cofilin‑1 (rat): S3‑p, Cofilin‑1 (pig): S3‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ILK (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion ILK (human) increase ILK adenovirus
fibronectin increase
LY294002 fibronectin no effect upon treatment-induced increase
PP2 fibronectin inhibit treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
p18_peptide fibronectin no effect upon treatment-induced increase
Y27632 fibronectin inhibit treatment-induced increase
fibronectin ILK (human) increase adenovirus ILK
siRNA fibronectin LIMK1 (human) ILK (human) no effect upon treatment-induced increase
fibronectin ROCK1 (rat) increase dominant negative
ROCK1 (rat) decrease cells in suspension
fibronectin ILK (human) increase
fibronectin TESK1 (rat) decrease
fibronectin TESK2 (rat) decrease
fibronectin ILK (human), TESK1 (rat) increase
fibronectin ILK (human), TESK2 (rat) increase
fibronectin ILK (human) increase SYF-/-
siRNA, fibronectin fibronectin LIMK1 (rat) ILK (human) inhibit treatment-induced increase SYF -/-
fibronectin increase
fibronectin, Y27632 fibronectin no effect upon treatment-induced increase
EGF Cofilin-1 (rat) decrease EGF treatment blocks S2 cofilin phosphorylation after cell adhesion.

Y397-p - FAK (rat)
Modsite: SVSETDDyAEIIDEE SwissProt Entrez-Gene
Orthologous residues
FAK (human): Y397‑p, FAK iso2 (human): Y216‑p, FAK iso5 (human): Y397‑p, FAK (mouse): Y397‑p, FAK iso2 (mouse): Y428‑p, FAK iso4 (mouse): Y397‑p, FAK iso9 (mouse): , FAK (rat): Y397‑p, FAK (chicken): Y397‑p, FAK iso5 (chicken):
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion ILK (human) increase ILK adenovirus
fibronectin increase
LY294002 fibronectin no effect upon treatment-induced increase
PP2 fibronectin inhibit treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
p18_peptide fibronectin no effect upon treatment-induced increase
Y27632 fibronectin inhibit treatment-induced increase
fibronectin ILK (human) increase
fibronectin TESK1 (rat) decrease
fibronectin TESK2 (rat) decrease
fibronectin ILK (human), TESK1 (rat) increase
fibronectin ILK (human), TESK2 (rat) increase
fibronectin ILK (human) increase SYF-/-
siRNA, fibronectin fibronectin LIMK1 (rat) ILK (human) inhibit treatment-induced increase SYF -/-
fibronectin increase
fibronectin, Y27632 fibronectin no effect upon treatment-induced increase

T508-p - LIMK1 (rat)
Modsite: PDRKKRYtVVGNPYW SwissProt Entrez-Gene
Orthologous residues
LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion ILK (human) increase ILK adenovirus

Y118-p - PXN (rat)
Modsite: AGEEEHVySFPNKQK SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p, PXN (cow): Y112‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cell_adhesion ILK (human) increase ILK adenovirus

Y419-p - Src (rat)
Modsite: RLIEDNEyTARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y418‑p, Src iso1 (mouse): Y424‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  RIE (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fibronectin increase
LY294002 fibronectin no effect upon treatment-induced increase
PP2 fibronectin inhibit treatment-induced increase
U0126 fibronectin no effect upon treatment-induced increase
p18_peptide fibronectin no effect upon treatment-induced increase
Y27632 fibronectin inhibit treatment-induced increase
fibronectin ILK (human) increase
fibronectin TESK1 (rat) decrease
fibronectin TESK2 (rat) decrease
fibronectin ILK (human), TESK1 (rat) increase
fibronectin ILK (human), TESK2 (rat) increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ILK (human) Induces molecular association, regulation pull-down assay, in vitro