Curated Information
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Home > Curated Information Page > PubMed Id: 18204460
Hosooka T, et al. (2008) Dok1 mediates high-fat diet-induced adipocyte hypertrophy and obesity through modulation of PPAR-gamma phosphorylation. Nat Med 14, 188-93 18204460
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S384-p - ELK1 (mouse)
Modsite: IHFWSTLsPIAPRsP SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S383‑p, ELK1 (mouse): S384‑p, ELK1 (rat): S382‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DOK1 (mouse) increase Dok1 -/-
U0126 DOK1 (mouse) decrease
SP600125 DOK1 (mouse) no change compared to control
DOK1 (mouse), PPAR-alpha (mouse) increase

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast) [DOK1 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U0126 no change compared to control
SP600125 decrease

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast) [DOK1 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
U0126 no change compared to control
SP600125 decrease

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast) [DOK1 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse

Y185-p - JNK2 (mouse)
Modsite: TNFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast) [DOK1 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse

S112-p - PPAR-gamma (mouse)
Modsite: AIkVEPAsPPYYSEK SwissProt Entrez-Gene
Orthologous residues
PPAR‑gamma (human): S112‑p, PPAR‑gamma iso2 (human): S84‑p, PPAR‑gamma iso3 (human): S84‑p, PPAR‑gamma (mouse): S112‑p, PPAR‑gamma iso2 (mouse): S82‑p, PPAR‑gamma (rat): S112‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast) [DOK1 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DOK1 (mouse) increase Dok1 -/-
U0126 DOK1 (mouse) decrease
SP600125 DOK1 (mouse) no change compared to control
Associated Diseases
Diseases Alterations Comments
obesity, hyperphagic decreased