Curated Information
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Home > Curated Information Page > PubMed Id: 15342649
Wang W, et al. (2004) AMP-activated protein kinase-regulated phosphorylation and acetylation of importin alpha1: involvement in the nuclear import of RNA-binding protein HuR. J Biol Chem 279, 48376-88 15342649
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K22-ac - KPNA2 (human)
Modsite: HRFKNkGkDsTEMRR SwissProt Entrez-Gene
Orthologous residues
KPNA2 (human): K22‑ac, KPNA2 (mouse): K22‑ac, KPNA2 (rat): K22‑ac
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial) [KPNA2 (human)], RKO (intestinal)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HuR (human) Induces intracellular localization co-immunoprecipitation
Comments:  promotes the nuclear localization of HuR

S105-p - KPNA2 (human)
Modsite: QAARkLLsREkQPPI SwissProt Entrez-Gene
Orthologous residues
KPNA2 (human): S105‑p, KPNA2 (mouse): S105‑p, KPNA2 (rat): S105‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial) [KPNA2 (human)], RKO (intestinal)
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (human) transfection of constitutively active enzyme
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
HuR (human) Induces intracellular localization co-immunoprecipitation
Comments:  promotes the nuclear localization of HuR