Curated Information
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Home > Curated Information Page > PubMed Id: 26152725
Das S, et al. (2015) Human Epidermal Growth Factor Receptor 2 (HER2) Impedes MLK3 Kinase Activity to Support Breast Cancer Cell Survival. J Biol Chem 290, 21705-12 26152725
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S674-p - MLK3 (human)
Modsite: PGRERGEsPTtPPTP SwissProt Entrez-Gene
Orthologous residues
MLK3 (human): S674‑p, MLK3 (mouse): S675‑p, MLK3 (rat): S675‑p, MLK3 (cow): Q674‑p
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  SKBr3 (breast cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human) transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-HER2 decrease
LY294002 anti-HER2 inhibit treatment-induced decrease
pictilisib anti-HER2 inhibit treatment-induced decrease
lapatinib decrease
HER2 (human) increase
siRNA HER2 (human) inhibit treatment-induced increase siRNA-HER2
Downstream Regulation
Effect of modification (function):  activity, inhibited
Effect of modification (process):  apoptosis, inhibited, carcinogenesis, induced