Curated Information
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Home > Curated Information Page > PubMed Id: 15304482
Li Y, et al. (2004) Dual phosphorylations underlie modulation of unitary KCNQ K(+) channels by Src tyrosine kinase. J Biol Chem 279, 45399-407 15304482
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y105-p - Kv7.3 (rat)
Modsite: VKRNNAKyRRIQTLI SwissProt Entrez-Gene
Orthologous residues
Kv7.3 (human): Y104‑p, Kv7.3 (mouse): Y105‑p, Kv7.3 iso3 (mouse): Y102‑p, Kv7.3 (rat): Y105‑p, Kv7.3 iso2 (rat): Y67‑p

Y387-p - Kv7.3 (rat)
Modsite: IQAAWRYyATNPNRL SwissProt Entrez-Gene
Orthologous residues
Kv7.3 (human): Y386‑p, Kv7.3 (mouse): Y387‑p, Kv7.3 iso3 (mouse): Y384‑p, Kv7.3 (rat): Y387‑p, Kv7.3 iso2 (rat): Y349‑p

Y67-p - Kv7.3 iso2 (rat)
Modsite: VKRNNAKyRRIQTLI SwissProt Entrez-Gene
Orthologous residues
Kv7.3 (human): Y104‑p, Kv7.3 (mouse): Y105‑p, Kv7.3 iso3 (mouse): Y102‑p, Kv7.3 (rat): Y105‑p, Kv7.3 iso2 (rat): Y67‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (rat) transfection of constitutively active enzyme, co-immunoprecipitation, phospho-motif antibody, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, inhibited
Comments:  inhibition of K(+) current via reducing open probability.

Y349-p - Kv7.3 iso2 (rat)
Modsite: IQAAWRYyATNPNRL SwissProt Entrez-Gene
Orthologous residues
Kv7.3 (human): Y386‑p, Kv7.3 (mouse): Y387‑p, Kv7.3 iso3 (mouse): Y384‑p, Kv7.3 (rat): Y387‑p, Kv7.3 iso2 (rat): Y349‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (rat) transfection of constitutively active enzyme, co-immunoprecipitation, phospho-motif antibody, transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  activity, inhibited
Comments:  inhibition of K(+) current via reducing open probability.