Curated Information
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Home > Curated Information Page > PubMed Id: 26091038
Wei J, et al. (2015) Glucose Uptake and Runx2 Synergize to Orchestrate Osteoblast Differentiation and Bone Formation. Cell 161, 1576-91 26091038
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T36-p - 4E-BP1 (mouse)
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GLUT1 (mouse) increase Glut1 knockout decreases

S79-p - ACC1 (mouse)
Modsite: FHMRSsMsGLHLVKQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GLUT1 (mouse) decrease Glut1 knockout increases
glucose decrease
GLUT1 (mouse) decrease GLUT1 knockout increases

T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GLUT1 (mouse) decrease Glut1 knockout increases
glucose decrease
GLUT1 (mouse) decrease GLUT1 knockout increases
glucose decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell differentiation, inhibited

S1149-p - eIF4G (mouse)
Modsite: VVQRssLsRERGEKA SwissProt Entrez-Gene
Orthologous residues
eIF4G (human): S1147‑p, eIF4G iso3 (human): S1107‑p, eIF4G iso8 (human): S1148‑p, eIF4G (mouse): S1149‑p, eIF4G iso2 (mouse): S1135‑p, eIF4G (rat): S1147‑p, eIF4G (rabbit): S950‑p, eIF4G (cow): S1174‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GLUT1 (mouse) increase Glut1 knockout decreases

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Raptor (mouse) increase RAPTOR knockout decreases
GLUT1 (mouse) increase GLUT1 knockout decreases
TSC1 (mouse) GLUT1 (mouse) inhibit treatment-induced increase TSC1/2 knockdown augments treatment induce increase
glucose AML3 (mouse) increase AML3 knockout

S792-p - Raptor (mouse)
Modsite: DKMRRVSsYSALNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GLUT1 (mouse) decrease Glut1 knockout increases

S148-p - SMURF1 (mouse)
Modsite: DRIGGGGsVVDCRGL SwissProt Entrez-Gene
Orthologous residues
SMURF1 (human): S148‑p, SMURF1 (mouse): S148‑p, SMURF1 (rat): S148‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  osteoblast-calvarium
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE AMPKA1 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE AMPKA1 (mouse) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose decrease
GLUT1 (mouse) decrease GLUT1 knockout increases
Downstream Regulation
Effect of modification (function):  protein degradation, ubiquitination
Effect of modification (process):  cell differentiation, inhibited
Comments:  ubiquitination and degradation of AML3