Whalley HJ, et al. (2015) Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation. Nat Commun 6, 7437 26078008

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T402-p - PAK2 (human) ▲

Modsite: PEQSkRstMVGtPYW SwissProt Entrez-Gene Orthologous residues PAK2 (human): T402‑p, PAK2 (mouse): T402‑p, PAK2 (rat): T402‑p, PAK2 (rabbit): T402‑p, PAK2 (cow): T402‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  MDCKII (epithelial) Cellular systems studied:  cell lines Species studied:  dog Downstream Regulation Effect of modification (function):  enzymatic activity, induced Effect of modification (process):  cell cycle regulation

S1466-p - TIAM1 (human) ▲

Modsite: SDAVsASsPEKESQQ SwissProt Entrez-Gene Orthologous residues TIAM1 (human): S1466‑p, TIAM1 (mouse): S1466‑p, TIAM1 (rat): S1465‑p Characterization Methods used to characterize site in vivo:  immunoassay, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), HEK293T (epithelial), MDCKII (epithelial), U2OS (bone cell) Cellular systems studied:  cell lines Species studied:  dog, human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK1 (human) phospho-motif antibody, modification site within consensus motif, pharmacological inhibitor of upstream enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes STLC increase in mitotic cells RO-3306 STLC inhibit treatment-induced increase monastrol increase increase followed by decrease upon monastrol release Downstream Regulation Effect of modification (function):  phosphorylation Effect of modification (process):  cell cycle regulation Comments:  induces phosphorylation and centrosomal activation of PAK1/2 in mitosis; regulates centrosome separation