Curated Information
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Home > Curated Information Page > PubMed Id: 25893286
Satija YK, Das S (2016) Tyr99 phosphorylation determines the regulatory milieu of tumor suppressor p73. Oncogene 35, 513-27 25893286
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y99-p - p73 (human)
Modsite: SVPTHSPyAQPSSTF SwissProt Entrez-Gene
Orthologous residues
p73 (human): Y99‑p, p73 iso2 (human): Y99‑p, p73 iso8 (human): Y50‑p, p73 iso10 (human): Y50‑p, p73 (mouse): Y91‑p
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal) [p53 (human), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
MG132 no change compared to control
etoposide MG132 increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation, ubiquitination
Effect of modification (process):  apoptosis, inhibited, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PCQAP (human) Induces co-immunoprecipitation
TIF1B (human) Disrupts pull-down assay
Comments:  PCQAP interacts with p73 only upon genotoxic stress, tumor suppressor and anti-metastatic functions