Curated Information
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Home > Curated Information Page > PubMed Id: 25860929
Li N, et al. (2015) FBXW7-mutated colorectal cancer cells exhibit aberrant expression of phosphorylated-p53 at Serine-15. Oncotarget 6, 9240-56 25860929
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T68-p - Chk2 (human)
Modsite: SSLETVStQELYSIP SwissProt Entrez-Gene
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  DLD1 (intestinal), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FBXW7 (human) decrease FBXW7 -/- slight increase

S516-p - Chk2 (human)
Modsite: PQVLAQPsTSRKRPR SwissProt Entrez-Gene
Orthologous residues
Chk2 (human): S516‑p, Chk2 iso9 (human): S559‑p, Chk2 iso11 (human): S198‑p, Chk2 iso12 (human): S487‑p, Chk2 (mouse): S520‑p, Chk2 (rat): S519‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  DLD1 (intestinal), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FBXW7 (human) decrease FBXW7 -/- slight increase

S15-p - p53 (human)
Modsite: PSVEPPLsQETFSDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  DLD1 (intestinal), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
FBXW7 (human) decrease RFBXW7 -/- induction
UV increase
UV FBXW7 (human) decrease FBXWW7 -/- angment increase
Associated Diseases
Diseases Alterations Comments
colorectal cancer increased FBXW7-mutations

S18-p - p53 iso2 (mouse)
Modsite: ISLELPLsQETFSGL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  DLD1 (intestinal), HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
increase fbxw7 G mice