Curated Information
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Home > Curated Information Page > PubMed Id: 10428862
Lock P, Casagranda F, Dunn AR (1999) Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck. J Biol Chem 274, 22775-84 10428862
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y276-p - DOK2 (mouse)
Modsite: LPRPESPySRPHDSL SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y271‑p, DOK2 (mouse): Y276‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [DOK2 (mouse)], HEK293T (epithelial) [LYN (mouse)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LYN (mouse) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (mouse) Induces co-immunoprecipitation

Y304-p - DOK2 (mouse)
Modsite: PGAPEGEyAVPFDTV SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y299‑p, DOK2 (mouse): Y304‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [DOK2 (mouse)], HEK293T (epithelial) [LYN (mouse)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LYN (mouse) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RASA1 (mouse) Induces co-immunoprecipitation

Y351-p - DOK2 (mouse)
Modsite: APLPDHIyDEPEGVA SwissProt Entrez-Gene
Orthologous residues
DOK2 (human): Y345‑p, DOK2 (mouse): Y351‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [DOK2 (mouse)], HEK293T (epithelial) [LYN (mouse)]
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LYN (mouse) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
NCK1 (mouse) Not reported co-immunoprecipitation