Curated Information
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Home > Curated Information Page > PubMed Id: 25978724
Liang L, et al. (2015) Endoplasmic reticulum stress impairs insulin receptor signaling in the brains of obese rats. PLoS One 10, e0126384 25978724
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S724-p - IRE1 (human)
Modsite: KLAVGRHsFsRRsGV SwissProt Entrez-Gene
Orthologous residues
IRE1 (human): S724‑p, IRE1 (mouse): S724‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, neuron-'brain, hippocampus', SH-SY5Y (neural crest)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase
insulin increase
tunicamycin insulin augment treatment-induced increase
thapsigargin insulin augment treatment-induced increase

S731-p - IRS2 (human)
Modsite: GGGYKAssPAEssPE SwissProt Entrez-Gene
Orthologous residues
IRS2 (human): S731‑p, IRS2 (mouse): S723‑p, IRS2 (rat): S724‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase in the hippocampus only

T183-p - JNK1 iso3 (human)
Modsite: AGTSFMMtPyVVTRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase
insulin increase
tunicamycin insulin augment treatment-induced increase
thapsigargin insulin augment treatment-induced increase

Y185-p - JNK1 iso3 (human)
Modsite: TSFMMtPyVVTRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase
insulin increase
tunicamycin insulin augment treatment-induced increase
thapsigargin insulin augment treatment-induced increase

S52-p - eIF2-alpha (rat)
Modsite: MILLSELsRRRIRSI SwissProt Entrez-Gene
Orthologous residues
eIF2‑alpha (human): S52‑p, eIF2‑alpha (mouse): S52‑p, eIF2‑alpha (rat): S52‑p, eIF2‑alpha (rabbit): S51‑p, eIF2‑alpha (fruit fly): S51‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, neuron-'brain, hippocampus', SH-SY5Y (neural crest)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase

S307-p - IRS1 (rat)
Modsite: TEsITATsPASMVGG SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S312‑p, IRS1 (mouse): S307‑p, IRS1 (rat): S307‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (rat) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
tunicamycin insulin augment treatment-induced increase
high-fat diet increase
high-fat diet IRE1 (human) augment treatment-induced increase siRNA decrease
thapsigargin increase
SP600125 high-fat diet inhibit treatment-induced increase

T974-p - PERK (rat)
Modsite: MPAYATHtGQVGTKL SwissProt Entrez-Gene
Orthologous residues
PERK (human): T982‑p, PERK (mouse): T980‑p, PERK (rat): T974‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, neuron-'brain, hippocampus', SH-SY5Y (neural crest)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
high-fat diet increase