Curated Information
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Home > Curated Information Page > PubMed Id: 25887626
Borgo C, et al. (2015) Protein kinase CK2 potentiates translation efficiency by phosphorylating eIF3j at Ser127. Biochim Biophys Acta 1853, 1693-701 25887626
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S129-p - Akt1 (human)
Modsite: sGsPsDNsGAEEMEV SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S129‑p, Akt1 iso2 (human): S67‑p, Akt1 (mouse): S129‑p, Akt1 (rat): S129‑p, Akt1 (fruit fly): E241‑p, Akt1 (cow): S129‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
silmitasertib decrease
quinalizarin decrease

S127-p - eIF3-alpha (human)
Modsite: LKkLQEEsDLELAkE SwissProt Entrez-Gene
Orthologous residues
eIF3‑alpha (human): S127‑p, eIF3‑alpha (mouse): S130‑p, eIF3‑alpha (rat): S128‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CK2A1 (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
silmitasertib decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  translation, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EIF3C (human) Induces co-immunoprecipitation
eIF3-zeta (human) Induces co-immunoprecipitation
eIF3-eta (human) Induces co-immunoprecipitation