Curated Information
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Home > Curated Information Page > PubMed Id: 25825709
Wang S, et al. (2015) α-Synuclein, a chemoattractant, directs microglial migration via H2O2-dependent Lyn phosphorylation. Proc Natl Acad Sci U S A 112, E1926-35 25825709
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y466-p - Cortactin (mouse)
Modsite: TyTSEPVyETTEAPG SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y470‑p, Cortactin iso2 (human): Y433‑p, Cortactin iso3 (human): Y433‑p, Cortactin (mouse): Y466‑p, Cortactin (rat): Y428‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  microglia
Cellular systems studied:  primary cultured cells
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LYN (mouse) siRNA inhibition of enzyme, activation of upstream enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  cell motility, induced, cytoskeletal reorganization
Comments:  relocalization of phosphorylated cortactin to the leading edge, microglial migration

Y397-p - LYN (mouse)
Modsite: RVIEDNEytAREGAK SwissProt Entrez-Gene
Orthologous residues
LYN (human): Y397‑p, LYN iso2 (human): Y376‑p, LYN (mouse): Y397‑p, LYN iso2 (mouse): Y376‑p, LYN (rat): Y397‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  microglia
Cellular systems studied:  primary cultured cells
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase