Yeung F, et al. (2015) Regulation of the mitogen-activated protein kinase kinase (MEK)-1 by NAD(+)-dependent deacetylases. Oncogene 34, 798-804 24681949

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K175-ac - MEK1 (human) ▲

Modsite: kVSIAVIkGLTYLRE SwissProt Entrez-Gene Orthologous residues MEK1 (human): K175‑ac, MEK1 iso2 (human): K149‑ac, MEK1 (mouse): K175‑ac, MEK1 (rat): K175‑ac, MEK1 (rabbit): K175‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT2 (human) co-immunoprecipitation, siRNA inhibition of enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme DEACETYLASE SIRT1 (human) siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  enzymatic activity, induced Effect of modification (process):  cell growth, induced, transcription, induced

S218-p - MEK1 (human) ▲

Modsite: VsGQLIDsMANsFVG SwissProt Entrez-Gene Orthologous residues MEK1 (human): S218‑p, MEK1 iso2 (human): S192‑p, MEK1 (mouse): S218‑p, MEK1 (rat): S218‑p, MEK1 (rabbit): S218‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S222-p - MEK1 (human) ▲

Modsite: LIDsMANsFVGtRSY SwissProt Entrez-Gene Orthologous residues MEK1 (human): S222‑p, MEK1 iso2 (human): S196‑p, MEK1 (mouse): S222‑p, MEK1 (rat): S222‑p, MEK1 (rabbit): S222‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  enzymatic activity, induced

K362-ac - MEK1 (human) ▲

Modsite: LMVHAFIkRSDAEEV SwissProt Entrez-Gene Orthologous residues MEK1 (human): K362‑ac, MEK1 iso2 (human): K336‑ac, MEK1 (mouse): K362‑ac, MEK1 (rat): K362‑ac, MEK1 (rabbit): K362‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes DEACETYLASE SIRT2 (human) co-immunoprecipitation, siRNA inhibition of enzyme ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme DEACETYLASE SIRT1 (human) siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  enzymatic activity, induced Effect of modification (process):  cell growth, induced, transcription, induced