Curated Information
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Home > Curated Information Page > PubMed Id: 18280244
Kurash JK, et al. (2008) Methylation of p53 by Set7/9 mediates p53 acetylation and activity in vivo. Mol Cell 29, 392-400 18280244
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K372-me - p53 (human)
Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑me, p53 iso2 (human): , p53 iso4 (human): K333‑me, p53 (mouse): K369‑me, p53 iso2 (mouse): , p53 (rat): K370‑me, p53 (rabbit): K370‑me, p53 (green monkey): K372‑me
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  bone marrow, HCT116 (intestinal), HEK293T (epithelial), MEF (fibroblast), spleen
Cellular systems studied:  cell lines, primary cultured cells, tissue
Species studied:  human, mouse
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Tip60 (mouse) Induces co-immunoprecipitation, in vitro

K117-ac - p53 (mouse)
Modsite: FLQSGTAkSVMCTYS SwissProt Entrez-Gene
Orthologous residues
p53 (human): K120‑ac, p53 iso2 (human): K120‑ac, p53 iso4 (human): K81‑ac, p53 (mouse): K117‑ac, p53 iso2 (mouse): K117‑ac, p53 (rat): K118‑ac, p53 (rabbit): K117‑ac, p53 (green monkey): K120‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
Relevant cell lines - cell types - tissues:  bone marrow
Cellular systems studied:  tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE Tip60 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SETD7 (mouse) increase

K317-ac - p53 (mouse)
Modsite: sAsPPQkkkPLDGEy SwissProt Entrez-Gene
Orthologous residues
p53 (human): K320‑ac, p53 iso2 (human): K320‑ac, p53 iso4 (human): K281‑ac, p53 (mouse): K317‑ac, p53 iso2 (mouse): K317‑ac, p53 (rat): K318‑ac, p53 (rabbit): K318‑ac, p53 (green monkey): K320‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
Relevant cell lines - cell types - tissues:  bone marrow
Cellular systems studied:  tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE PCAF (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SETD7 (mouse) increase

K369-me - p53 (mouse)
Modsite: HSSYLkTkkGQsTsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑me, p53 iso2 (human): , p53 iso4 (human): K333‑me, p53 (mouse): K369‑me, p53 iso2 (mouse): , p53 (rat): K370‑me, p53 (rabbit): K370‑me, p53 (green monkey): K372‑me
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
Relevant cell lines - cell types - tissues:  bone marrow, MEF (fibroblast), spleen
Cellular systems studied:  cell lines, primary cultured cells, tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
METHYLTRANSFERASE SETD7 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
METHYLTRANSFERASE SETD7 (mouse) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  acetylation
Comments:  increases p53 acetylation at multiple sites

K370-ac - p53 (mouse)
Modsite: SSYLkTkkGQsTsRH SwissProt Entrez-Gene
Orthologous residues
p53 (human): K373‑ac, p53 iso2 (human): , p53 iso4 (human): K334‑ac, p53 (mouse): K370‑ac, p53 iso2 (mouse): , p53 (rat): K371‑ac, p53 (rabbit): K371‑ac, p53 (green monkey): K373‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
Relevant cell lines - cell types - tissues:  bone marrow
Cellular systems studied:  tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SETD7 (mouse) increase

K379-ac - p53 (mouse)
Modsite: QsTsRHkkTMVkkVG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ac, p53 iso2 (human): , p53 iso4 (human): K343‑ac, p53 (mouse): K379‑ac, p53 iso2 (mouse): , p53 (rat): K380‑ac, p53 (rabbit): K380‑ac, p53 (green monkey): K382‑ac
Characterization
Methods used to characterize site in vivo immunoprecipitation, modification-specific antibody, western blotting
Relevant cell lines - cell types - tissues:  bone marrow, MEF (fibroblast), spleen
Cellular systems studied:  cell lines, primary cultured cells, tissue
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
ACETYLTRANSFERASE p300 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SETD7 (mouse) increase