Curated Information
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Home > Curated Information Page > PubMed Id: 18695000
Wee HJ, Voon DC, Bae SC, Ito Y (2008) PEBP2-beta/CBF-beta-dependent phosphorylation of RUNX1 and p300 by HIPK2: implications for leukemogenesis. Blood 112, 3777-87 18695000
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S249-p - AML1 (human)
Modsite: DTRQIQPsPPWsyDQ SwissProt Entrez-Gene
Orthologous residues
AML1 (human): S249‑p, AML1 iso2 (human): S249‑p, AML1 iso8 (human): S276‑p, AML1 iso10 (human): S264‑p, AML1 (mouse): S249‑p, AML1 (rat): S249‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE HIPK2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DYRK1A (human) increase
MYH11 (human) decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBFB (human) Induces electrophoretic visualization
Comments:  promotes AML1 and p300 phosphorylation

S266-p - AML1 (human)
Modsite: QYLGsIAsPsVHPAt SwissProt Entrez-Gene
Orthologous residues
AML1 (human): S266‑p, AML1 iso2 (human): S266‑p, AML1 iso8 (human): S293‑p, AML1 iso10 (human): S281‑p, AML1 (mouse): S266‑p, AML1 (rat): S266‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBFB (human) Induces electrophoretic visualization
Comments:  promotes AML1 and p300 phosphorylation

T273-p - AML1 (human)
Modsite: sPsVHPAtPIsPGRA SwissProt Entrez-Gene
Orthologous residues
AML1 (human): T273‑p, AML1 iso2 (human): T273‑p, AML1 iso8 (human): T300‑p, AML1 iso10 (human): T288‑p, AML1 (mouse): T273‑p, AML1 (rat): T272‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE HIPK2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DYRK1A (human) increase
MYH11 (human) decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation, phosphorylation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CBFB (human) Induces electrophoretic visualization
Comments:  promotes AML1 and p300 phosphorylation

S276-p - AML1 (human)
Modsite: VHPAtPIsPGRASGM SwissProt Entrez-Gene
Orthologous residues
AML1 (human): S276‑p, AML1 iso2 (human): S276‑p, AML1 iso8 (human): S303‑p, AML1 iso10 (human): S291‑p, AML1 (mouse): S276‑p, AML1 (rat): S275‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE HIPK2 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
DYRK1A (human) increase
MYH11 (human) decrease

S831-p - p300 (human)
Modsite: QPALHQNsPsPVPSR SwissProt Entrez-Gene
Orthologous residues
p300 (human): S831‑p, p300 (mouse): S831‑p, p300 (rat): S831‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

S833-p - p300 (human)
Modsite: ALHQNsPsPVPSRtP SwissProt Entrez-Gene
Orthologous residues
p300 (human): S833‑p, p300 (mouse): S833‑p, p300 (rat): S833‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

T839-p - p300 (human)
Modsite: PsPVPSRtPtPHHtP SwissProt Entrez-Gene
Orthologous residues
p300 (human): T839‑p, p300 (mouse): T839‑p, p300 (rat): T839‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

T841-p - p300 (human)
Modsite: PVPSRtPtPHHtPPS SwissProt Entrez-Gene
Orthologous residues
p300 (human): T841‑p, p300 (mouse): T841‑p, p300 (rat): T841‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

T845-p - p300 (human)
Modsite: RtPtPHHtPPSIGAQ SwissProt Entrez-Gene
Orthologous residues
p300 (human): T845‑p, p300 (mouse): T845‑p, p300 (rat): T845‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

T885-p - p300 (human)
Modsite: LHPPPRQtPtPPTtQ SwissProt Entrez-Gene
Orthologous residues
p300 (human): T885‑p, p300 (mouse): T885‑p, p300 (rat): T885‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase

T887-p - p300 (human)
Modsite: PPPRQtPtPPTtQLP SwissProt Entrez-Gene
Orthologous residues
p300 (human): T887‑p, p300 (mouse): T887‑p, p300 (rat): T887‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial), COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
AML1 (human) increase