Curated Information
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Home > Curated Information Page > PubMed Id: 18541536
Granum S, et al. (2008) Modulation of Lck function through multisite docking to T cell-specific adapter protein. J Biol Chem 283, 21909-19 18541536
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y394-p - Lck (human)
Modsite: RLIEDNEytAREGAk SwissProt Entrez-Gene
Orthologous residues
Lck (human): Y394‑p, Lck iso3 (human): Y424‑p, Lck (mouse): Y394‑p, Lck (rat): Y394‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-TCR increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Lck (human) SH2 Induces pull-down assay

Y260-p - TSAd (human)
Modsite: PQLPPEVytIPVPRH SwissProt Entrez-Gene
Orthologous residues
TSAd (human): Y260‑p, TSAd iso4 (human): Y242‑p, TSAd (mouse): Y283‑p

Y280-p - TSAd (human)
Modsite: PKPSNPIyNEPDEPI SwissProt Entrez-Gene
Orthologous residues
TSAd (human): Y280‑p, TSAd iso4 (human): Y262‑p, TSAd (mouse): Y300‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Lck (human) SH2 Induces pull-down assay

Y290-p - TSAd (human)
Modsite: PDEPIAFyAMGrGsP SwissProt Entrez-Gene
Orthologous residues
TSAd (human): Y290‑p, TSAd iso4 (human): Y272‑p, TSAd (mouse): Y310‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Lck (human) SH2 Induces pull-down assay

Y305-p - TSAd (human)
Modsite: GEAPsNIyVEVEDEG SwissProt Entrez-Gene
Orthologous residues
TSAd (human): Y305‑p, TSAd iso4 (human): Y287‑p, TSAd (mouse): Y325‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Lck (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Lck (human) SH2 Induces pull-down assay