Curated Information
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Home > Curated Information Page > PubMed Id: 25815641
Herren AW, et al. (2015) CaMKII Phosphorylation of Na(V)1.5: Novel in Vitro Sites Identified by Mass Spectrometry and Reduced S516 Phosphorylation in Human Heart Failure. J Proteome Res 14, 2298-311 25815641
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S11-p - SCN5A (human)
Modsite: FLLPRGTssFRRFtR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S11‑p, SCN5A (mouse): S11‑p, SCN5A (rat): S11‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S12-p - SCN5A (human)
Modsite: LLPRGTssFRRFtRE SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S12‑p, SCN5A (mouse): S12‑p, SCN5A (rat): S12‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

T17-p - SCN5A (human)
Modsite: TssFRRFtREsLAAI SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): T17‑p, SCN5A (mouse): T17‑p, SCN5A (rat): T17‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S20-p - SCN5A (human)
Modsite: FRRFtREsLAAIEKR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S20‑p, SCN5A (mouse): S20‑p, SCN5A (rat): S20‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S42-p - SCN5A (human)
Modsite: GSTTLQEsREGLPEE SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S42‑p, SCN5A (mouse): S42‑p, SCN5A (rat): S43‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S61-p - SCN5A (human)
Modsite: PQLDLQAsKKLPDLY SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S61‑p, SCN5A (mouse): S61‑p, SCN5A (rat): S62‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

T455-p - SCN5A (human)
Modsite: LTIRGVDtVsRssLE SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): T455‑p, SCN5A (mouse): T455‑p, SCN5A (rat): T456‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S457-p - SCN5A (human)
Modsite: IRGVDtVsRssLEMs SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S457‑p, SCN5A (mouse): S457‑p, SCN5A (rat): S458‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S460-p - SCN5A (human)
Modsite: VDtVsRssLEMsPLA SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S460‑p, SCN5A (mouse): S460‑p, SCN5A (rat): S461‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S464-p - SCN5A (human)
Modsite: sRssLEMsPLAPVNs SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S464‑p, SCN5A (mouse): S464‑p, SCN5A (rat): S465‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S471-p - SCN5A (human)
Modsite: sPLAPVNsHERRsKR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S471‑p, SCN5A (mouse): N471‑p, SCN5A (rat): N472‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S483-p - SCN5A (human)
Modsite: sKRRKRMssGtEECG SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S483‑p, SCN5A (mouse): S483‑p, SCN5A (rat): S484‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S484-p - SCN5A (human)
Modsite: KRRKRMssGtEECGE SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S484‑p, SCN5A (mouse): S484‑p, SCN5A (rat): S485‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S497-p - SCN5A (human)
Modsite: GEDRLPKsDsEDGPR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S497‑p, SCN5A (mouse): S497‑p, SCN5A (rat): S498‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S499-p - SCN5A (human)
Modsite: DRLPKsDsEDGPRAM SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S499‑p, SCN5A (mouse): S499‑p, SCN5A (rat): S500‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S510-p - SCN5A (human)
Modsite: PRAMNHLsLTrGLsR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S510‑p, SCN5A (mouse): S510‑p, SCN5A (rat): S511‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S516-p - SCN5A (human)
Modsite: LsLTrGLsRTsMKPR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S516‑p, SCN5A (mouse): S516‑p, SCN5A (rat): S517‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), heart
Cellular systems studied:  cell lines, tissue
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMK2A (human) phospho-antibody
Associated Diseases
Diseases Alterations Comments
DCM, cardiomyopathy decreased

S528-p - SCN5A (human)
Modsite: KPRssrGsIFtFRRR SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S528‑p, SCN5A (mouse): S528‑p, SCN5A (rat): S529‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S539-p - SCN5A (human)
Modsite: FRRRDLGsEADFADD SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S539‑p, SCN5A (mouse): S539‑p, SCN5A (rat): S540‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

T570-p - SCN5A (human)
Modsite: VPWPLRRtsAQGQPs SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): T570‑p, SCN5A (mouse): P570‑p, SCN5A (rat): P571‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S571-p - SCN5A (human)
Modsite: PWPLRRtsAQGQPsP SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S571‑p, SCN5A (mouse): S571‑p, SCN5A (rat): S572‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S577-p - SCN5A (human)
Modsite: tsAQGQPsPGTSAPG SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S577‑p, SCN5A (mouse): G577‑p, SCN5A (rat): G578‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S664-p - SCN5A (human)
Modsite: GARQRALsAVsVLts SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S664‑p, SCN5A (mouse): S664‑p, SCN5A (rat): S665‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S667-p - SCN5A (human)
Modsite: QRALsAVsVLtsALE SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S667‑p, SCN5A (mouse): S667‑p, SCN5A (rat): S668‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1003-p - SCN5A (human)
Modsite: AAQGQLPsCIATPYS SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1003‑p, SCN5A (mouse): S1005‑p, SCN5A (rat): S1006‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1503-p - SCN5A (human)
Modsite: NAMKKLGsKKPQKPI SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1503‑p, SCN5A (mouse): S1505‑p, SCN5A (rat): S1505‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1865-p - SCN5A (human)
Modsite: TKRVLGEsGEMDALK SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1865‑p, SCN5A (mouse): S1867‑p, SCN5A (rat): S1867‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1885-p - SCN5A (human)
Modsite: KFMAANPsKISYEPI SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1885‑p, SCN5A (mouse): S1887‑p, SCN5A (rat): S1887‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1920-p - SCN5A (human)
Modsite: RRHLLQRsLKHAsFL SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1920‑p, SCN5A (mouse): S1922‑p, SCN5A (rat): S1922‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1925-p - SCN5A (human)
Modsite: QRsLKHAsFLFRQQA SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1925‑p, SCN5A (mouse): S1927‑p, SCN5A (rat): S1927‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1934-p - SCN5A (human)
Modsite: LFRQQAGsGLsEEDA SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1934‑p, SCN5A (mouse): S1936‑p, SCN5A (rat): G1936‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1937-p - SCN5A (human)
Modsite: QQAGsGLsEEDAPER SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1937‑p, SCN5A (mouse): S1940‑p, SCN5A (rat): S1940‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S1998-p - SCN5A (human)
Modsite: RGSDYSHsEDLADFP SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S1998‑p, SCN5A (mouse): S2001‑p, SCN5A (rat): S2001‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)

S2007-p - SCN5A (human)
Modsite: DLADFPPsPDRDRES SwissProt Entrez-Gene
Orthologous residues
SCN5A (human): S2007‑p, SCN5A (mouse): S2010‑p, SCN5A (rat): S2010‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2A (human)