Curated Information
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Home > Curated Information Page > PubMed Id: 25755284
Gong J, et al. (2015) The C2 Domain and Altered ATP-Binding Loop Phosphorylation at Ser³⁵⁹ Mediate the Redox-Dependent Increase in Protein Kinase C-δ Activity. Mol Cell Biol 35, 1727-40 25755284
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T295-p - PKCD (human)
Modsite: AEALNQVtQRAsRRs SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T295‑p, PKCD iso2 (human): T295‑p, PKCD (mouse): T295‑p, PKCD iso2 (mouse): T295‑p, PKCD (rat): T295‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCD (human)

Y313-p - PKCD (human)
Modsite: ssEPVGIyQGFEKkT SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y313‑p, PKCD iso2 (human): Y313‑p, PKCD (mouse): Y311‑p, PKCD iso2 (mouse): Y311‑p, PKCD (rat): Y311‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Src (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
phorbol_ester increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, altered, molecular association, regulation, protein conformation
Comments:  interaction between phosphorylated hinge region and its phosphotyrosine-binding C2 domain which indirectly by decreasing phosphorylation in the kinase domain ATP-positioning loop at Ser359.

Y334-p - PKCD (human)
Modsite: MQDNsGtyGkIWEGs SwissProt Entrez-Gene
Orthologous residues
PKCD (human): Y334‑p, PKCD iso2 (human): Y365‑p, PKCD (mouse): Y332‑p, PKCD iso2 (mouse): Y358‑p, PKCD (rat): Y332‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester no change compared to control
H2O2 no change compared to control

S359-p - PKCD (human)
Modsite: HKVLGkGsFGkVLLG SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S359‑p, PKCD iso2 (human): S390‑p, PKCD (mouse): S357‑p, PKCD iso2 (mouse): S383‑p, PKCD (rat): S357‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, altered
Comments:  Y313 indirectly decreases phosphorylation in the kinase domain ATP-positioning loop at Ser359

T507-p - PKCD (human)
Modsite: FGEsRAstFCGtPDy SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester no change compared to control
H2O2 no change compared to control

S645-p - PKCD (human)
Modsite: LNEkARLsysDKNLI SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S645‑p, PKCD iso2 (human): S676‑p, PKCD (mouse): S643‑p, PKCD iso2 (mouse): S669‑p, PKCD (rat): S643‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester no change compared to control
H2O2 no change compared to control

S664-p - PKCD (human)
Modsite: QsAFAGFsFVNPKFE SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S664‑p, PKCD iso2 (human): S695‑p, PKCD (mouse): S662‑p, PKCD iso2 (mouse): S688‑p, PKCD (rat): S662‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester no change compared to control
H2O2 no change compared to control

S23-p - TNNI3 (human)
Modsite: PAPIRRRssNyRAyA SwissProt Entrez-Gene
Orthologous residues
TNNI3 (human): S23‑p, TNNI3 (mouse): S23‑p, TNNI3 (rat): S23‑p, TNNI3 (rabbit): S22‑p, TNNI3 (dog): S23‑p, TNNI3 (cow): S24‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S24-p - TNNI3 (human)
Modsite: APIRRRssNyRAyAt SwissProt Entrez-Gene
Orthologous residues
TNNI3 (human): S24‑p, TNNI3 (mouse): S24‑p, TNNI3 (rat): S24‑p, TNNI3 (rabbit): S23‑p, TNNI3 (dog): S24‑p, TNNI3 (cow): S25‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T143-p - TNNI3 (human)
Modsite: RGKFKRPtLRRVrIs SwissProt Entrez-Gene
Orthologous residues
TNNI3 (human): T143‑p, TNNI3 (mouse): T144‑p, TNNI3 (rat): T144‑p, TNNI3 (rabbit): T143‑p, TNNI3 (dog): T144‑p, TNNI3 (cow): T145‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human