Zhao J, et al. (2013) SCF E3 ligase F-box protein complex SCF(FBXL19) regulates cell migration by mediating Rac1 ubiquitination and degradation. FASEB J 27, 2611-9 23512198

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S71-p - RAC1 (human) ▲

Modsite: yDRLRPLsYPQTDVF SwissProt Entrez-Gene Orthologous residues RAC1 (human): S71‑p, RAC1 iso2 (human): S71‑p, RAC1 (mouse): S71‑p, RAC1 (rat): S71‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MLE12 (epithelial) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Akt1 (human) increase Downstream Regulation Effect of modification (function):  protein degradation Comments:  Akt induces Rac1 degradation via S71 phosphorylation

K166-ub - RAC1 (human) ▲

Modsite: ALtQrGLktVFDEAI SwissProt Entrez-Gene Orthologous residues RAC1 (human): K166‑ub, RAC1 iso2 (human): K185‑ub, RAC1 (mouse): K166‑ub, RAC1 (rat): K166‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  MLE12 (epithelial) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE FBXL19 (human) co-immunoprecipitation, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes Akt1 (human) increase Downstream Regulation Effect of modification (function):  protein degradation Effect of modification (process):  cell motility, inhibited Comments:  Akt induces Rac1 degradation via S71 phosphorylation