Curated Information
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Home > Curated Information Page > PubMed Id: 10521487
Bertrand L, et al. (1999) Heart 6-phosphofructo-2-kinase activation by insulin results from Ser-466 and Ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase B. J Biol Chem 274, 30927-33 10521487
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S467-p - PFKFB2 (cow)
Modsite: PVRMRRNsFTPLSSS SwissProt Entrez-Gene
Orthologous residues
PFKFB2 (human): S466‑p, PFKFB2 iso2 (human): , PFKFB2 (mouse): S469‑p, PFKFB2 iso3 (mouse): S468‑p, PFKFB2 (rat): S469‑p, PFKFB2 (cow): S467‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin, insulin insulin inhibit treatment-induced increase

S484-p - PFKFB2 (cow)
Modsite: IRRPRNYsVGSRPLQ SwissProt Entrez-Gene
Orthologous residues
PFKFB2 (human): S483‑p, PFKFB2 iso2 (human): , PFKFB2 (mouse): S486‑p, PFKFB2 iso3 (mouse): S485‑p, PFKFB2 (rat): S486‑p, PFKFB2 (cow): S484‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
wortmannin, insulin insulin inhibit treatment-induced increase

S494-p - PFKFB2 (cow)
Modsite: SRPLQPLsPLRALDT SwissProt Entrez-Gene
Orthologous residues
PFKFB2 (human): S493‑p, PFKFB2 iso2 (human): , PFKFB2 (mouse): S496‑p, PFKFB2 iso3 (mouse): S495‑p, PFKFB2 (rat): S496‑p, PFKFB2 (cow): S494‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S523-p - PFKFB2 (cow)
Modsite: RAAHRLPsPAPPTsP SwissProt Entrez-Gene
Orthologous residues
PFKFB2 (human): , PFKFB2 iso2 (human): S463‑p, PFKFB2 (mouse): , PFKFB2 iso3 (mouse): , PFKFB2 (rat): S549‑p, PFKFB2 (cow): S523‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S529-p - PFKFB2 (cow)
Modsite: PsPAPPTsPS_____ SwissProt Entrez-Gene
Orthologous residues
PFKFB2 (human): , PFKFB2 iso2 (human): L469‑p, PFKFB2 (mouse): P517‑p, PFKFB2 iso3 (mouse): P516‑p, PFKFB2 (rat): L555‑p, PFKFB2 (cow): S529‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, peptide sequencing, phosphoamino acid analysis
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human