Curated Information
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Home > Curated Information Page > PubMed Id: 25704810
Lee KG, et al. (2015) Bruton's Tyrosine Kinase Phosphorylates DDX41 and Activates Its Binding of dsDNA and STING to Initiate Type 1 Interferon Response. Cell Rep 10, 1055-65 25704810
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y223-p - Btk (human)
Modsite: LKKVVALyDyMPMNA SwissProt Entrez-Gene
Orthologous residues
Btk (human): Y223‑p, Btk iso2 (human): Y257‑p, Btk (mouse): Y223‑p, Btk (rat): Y224‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-bone marrow
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
cGMP_analog increase
DMXAA increase

Y414-p - DDX41 (human)
Modsite: DVIQEVEyVkEEAKM SwissProt Entrez-Gene
Orthologous residues
DDX41 (human): Y414‑p, DDX41 (mouse): Y414‑p, DDX41 (rat): Y414‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), macrophage-bone marrow
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Btk (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Btk (human) transfection of wild-type enzyme, phosphopeptide analysis, transfection of inactive enzyme, co-immunoprecipitation, transfection of constitutively active enzyme
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces co-immunoprecipitation
STING (human) Induces co-immunoprecipitation