Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.7.7
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 25670854
Nakajima T, et al. (2015) Regulation of GATA-binding protein 2 levels via ubiquitin-dependent degradation by Fbw7: involvement of cyclin B-cyclin-dependent kinase 1-mediated phosphorylation of THR176 in GATA-binding protein 2. J Biol Chem 290, 10368-81 25670854
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T176-p - GATA2 (human)
Modsite: HLFGFPPtPPKEVsP SwissProt Entrez-Gene
Orthologous residues
GATA2 (human): T176‑p, GATA2 (mouse): T176‑p, GATA2 (rat): T176‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Cellular systems studied:  cell lines
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, modification site within consensus motif
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein degradation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FBXW7 (human) Induces co-immunoprecipitation