Curated Information
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Home > Curated Information Page > PubMed Id: 25568349
Bartels M, et al. (2015) Acetylation of C/EBPε is a prerequisite for terminal neutrophil differentiation. Blood 125, 1782-92 25568349
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K121-ac - C/EBP-epsilon (human)
Modsite: DPRAVAVkEEPRGPE SwissProt Entrez-Gene
Orthologous residues
C/EBP‑epsilon (human): K121‑ac, C/EBP‑epsilon (mouse): K121‑ac, C/EBP‑epsilon (rat): K121‑ac
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  leukemia, acute myelogenous leukemia
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid), NB-4 (myeloid), neutrophil
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
DEACETYLASE SIRT1 (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
valproic acid no change compared to control
nicotinamide increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell differentiation, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces

K198-ac - C/EBP-epsilon (human)
Modsite: GPLHKGKkAVNKDSL SwissProt Entrez-Gene
Orthologous residues
C/EBP‑epsilon (human): K198‑ac, C/EBP‑epsilon (mouse): K198‑ac, C/EBP‑epsilon (rat): K198‑ac
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mass spectrometry, modification-specific antibody, mutation of modification site, western blotting
Disease tissue studied:  leukemia, acute myelogenous leukemia
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid), NB-4 (myeloid), neutrophil
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
ACETYLTRANSFERASE p300 (human) transfection of wild-type enzyme
DEACETYLASE SIRT1 (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
valproic acid no change compared to control
nicotinamide increase
Downstream Regulation
Effect of modification (process):  cell differentiation, induced, transcription, induced