Yu Z, et al. (2015) Dynamic Phosphorylation of CENP-A at Ser68 Orchestrates Its Cell-Cycle-Dependent Deposition at Centromeres. Dev Cell 32, 68-81 25556658

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S68-p - CENPA (human) ▲

Modsite: LIRKLPFsRLAREIC SwissProt Entrez-Gene Orthologous residues CENPA (human): S68‑p, CENPA (mouse): S62‑p, CENPA (rat): G87‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK1 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP1CA (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, co-immunoprecipitation KINASE CDK1 (human) pharmacological inhibitor of upstream enzyme, siRNA inhibition of enzyme, co-immunoprecipitation Downstream Regulation Comments:  regulation of CENPA loading onto centromeric chromatin, recognition of CENPA and centromere integrity during mitosis