Curated Information
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Home > Curated Information Page > PubMed Id: 8106527
Middlemas DS, Meisenhelder J, Hunter T (1994) Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor. J Biol Chem 269, 5458-66 8106527
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y771-p - PLCG1 (rat)
Modsite: IGTAEPDyGALyEGR SwissProt Entrez-Gene
Orthologous residues
PLCG1 (human): Y771‑p, PLCG1 iso2 (human): Y771‑p, PLCG1 (mouse): Y771‑p, PLCG1 (rat): Y771‑p, PLCG1 (cow): Y771‑p
Characterization
Methods used to characterize site in vivo phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

Y775-p - PLCG1 (rat)
Modsite: EPDyGALyEGRNPGF SwissProt Entrez-Gene
Orthologous residues
PLCG1 (human): Y775‑p, PLCG1 iso2 (human): Y775‑p, PLCG1 (mouse): Y775‑p, PLCG1 (rat): Y775‑p, PLCG1 (cow): Y775‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat

Y783-p - PLCG1 (rat)
Modsite: EGRNPGFyVEANPMP SwissProt Entrez-Gene
Orthologous residues
PLCG1 (human): Y783‑p, PLCG1 iso2 (human): Y783‑p, PLCG1 (mouse): Y783‑p, PLCG1 (rat): Y783‑p, PLCG1 (cow): Y783‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase

Y1253-p - PLCG1 (rat)
Modsite: EGsFEARyQQPFEDF SwissProt Entrez-Gene
Orthologous residues
PLCG1 (human): Y1253‑p, PLCG1 iso2 (human): Y1254‑p, PLCG1 (mouse): Y1253‑p, PLCG1 (rat): Y1253‑p, PLCG1 (cow): Y1254‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase

Y701-p - TrkB (rat)
Modsite: FGMSRDVyStDyyRV SwissProt Entrez-Gene
Orthologous residues
TrkB (human): Y702‑p, TrkB iso4 (human): Y718‑p, TrkB (mouse): Y701‑p, TrkB iso2 (mouse): , TrkB (rat): Y701‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphoaminoacid analysis, phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase

Y705-p - TrkB (rat)
Modsite: RDVyStDyyRVGGHT SwissProt Entrez-Gene
Orthologous residues
TrkB (human): Y706‑p, TrkB iso4 (human): Y722‑p, TrkB (mouse): Y705‑p, TrkB iso2 (mouse): , TrkB (rat): Y705‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphoaminoacid analysis, phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase

Y706-p - TrkB (rat)
Modsite: DVyStDyyRVGGHTM SwissProt Entrez-Gene
Orthologous residues
TrkB (human): Y707‑p, TrkB iso4 (human): Y723‑p, TrkB (mouse): Y706‑p, TrkB iso2 (mouse): , TrkB (rat): Y706‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphoaminoacid analysis, phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase

Y816-p - TrkB (rat)
Modsite: LAkAsPVyLDILG__ SwissProt Entrez-Gene
Orthologous residues
TrkB (human): Y817‑p, TrkB iso4 (human): Y833‑p, TrkB (mouse): Y816‑p, TrkB iso2 (mouse): , TrkB (rat): Y816‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  Rat2 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TrkB (rat) phosphoaminoacid analysis, phosphopeptide analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
neurotrophin-3 increase