Curated Information
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Home > Curated Information Page > PubMed Id: 25466889
Hammer A, Oladimeji P, De Las Casas LE, Diakonova M (2015) Phosphorylation of tyrosine 285 of PAK1 facilitates ╬▓PIX/GIT1 binding and adhesion turnover. FASEB J 29, 943-59 25466889
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y153-p - PAK1 (human)
Modsite: tDksAEDyNssNALN SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): Y153‑p, PAK1 (mouse): Y153‑p, PAK1 (rat): Y153‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  breast cell-breast, HEK293T (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GIT1 (human) Induces co-immunoprecipitation
ARHGEF7 (human) SH3 Induces co-immunoprecipitation
Comments:  impairs cell adhesion, alters distribution of adhesion complex

Y201-p - PAK1 (human)
Modsite: PEHtKsVyTRsVIEP SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): Y201‑p, PAK1 (mouse): Y201‑p, PAK1 (rat): Y200‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  breast cell-breast, HEK293T (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GIT1 (human) Induces co-immunoprecipitation
ARHGEF7 (human) SH3 Induces co-immunoprecipitation
Comments:  impairs cell adhesion, alters distribution of adhesion complex

Y285-p - PAK1 (human)
Modsite: QGASGTVyTAMDVAT SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): Y285‑p, PAK1 (mouse): Y285‑p, PAK1 (rat): Y284‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  breast cell-breast, HEK293T (epithelial), MCF-7 (breast cell), T47D (breast cell)
Cellular systems studied:  cell lines, tissue
Species studied:  human
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARHGEF7 (human) SH3 Induces co-immunoprecipitation
GIT1 (human) Induces co-immunoprecipitation
PXN (human) Induces microscopy-colocalization
Comments:  impairs cell adhesion, alters distribution of adhesion complex, regulation of adhesion turnover
Associated Diseases
Diseases Alterations Comments
breast ductal carcinoma increased

T423-p - PAK1 (human)
Modsite: PEQSkRstMVGtPYW SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): T423‑p, PAK1 (mouse): T423‑p, PAK1 (rat): T422‑p
Downstream Regulation
Effect of modification (function):  activity, induced

T402-p - PAK2 (human)
Modsite: PEQSkRstMVGtPYW SwissProt Entrez-Gene
Orthologous residues
PAK2 (human): T402‑p, PAK2 (mouse): T402‑p, PAK2 (rat): T402‑p, PAK2 (rabbit): T402‑p, PAK2 (cow): T402‑p
Downstream Regulation
Effect of modification (function):  activity, induced

Y31-p - PXN (human)
Modsite: FLSEEtPysyPtGNH SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y31‑p, PXN iso2 (human): Y31‑p, PXN iso3 (human): Y31‑p, PXN (mouse): Y31‑p, PXN iso2 (mouse): Y31‑p, PXN (rat): Y31‑p, PXN (chicken): Y31‑p, PXN (cow): Y26‑p

Y118-p - PXN (human)
Modsite: VGEEEHVysFPNkQk SwissProt Entrez-Gene
Orthologous residues
PXN (human): Y118‑p, PXN iso2 (human): Y118‑p, PXN iso3 (human): Y118‑p, PXN (mouse): Y118‑p, PXN iso2 (mouse): Y118‑p, PXN (rat): Y118‑p, PXN (chicken): Y118‑p, PXN (cow): Y112‑p

S272-p - PXN (human)
Modsite: ELDELMAsLsDFkIQ SwissProt Entrez-Gene
Orthologous residues
PXN (human): S272‑p, PXN iso2 (human): S272‑p, PXN iso3 (human): S272‑p, PXN (mouse): S272‑p, PXN iso2 (mouse): S272‑p, PXN (rat): S301‑p, PXN (chicken): S273‑p, PXN (cow): S266‑p