Curated Information
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Home > Curated Information Page > PubMed Id: 25527497
Aerts L, Craessaerts K, De Strooper B, Morais VA (2015) PINK1 Kinase Catalytic Activity Is Regulated by Phosphorylation on Serines 228 and 402. J Biol Chem 290, 2798-811 25527497
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S228-p - PINK1 (human)
Modsite: MWNISAGsSSEAILN SwissProt Entrez-Gene
Orthologous residues
PINK1 (human): S228‑p, PINK1 (mouse): S227‑p, PINK1 (rat): S227‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HEK293T (epithelial), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PINK1 (human)
Comments:  Autophosphorylation on truncated PINK1
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lactacystin increase
CCCP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  T313A and S402A mutants interfere with PINK1 phosphorylation; regulate substrate phosphorylation, Parkin and Ubiquitin

S402-p - PINK1 (human)
Modsite: GLQLPFSsWYVDRGG SwissProt Entrez-Gene
Orthologous residues
PINK1 (human): S402‑p, PINK1 (mouse): S401‑p, PINK1 (rat): S401‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HEK293T (epithelial), HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PINK1 (human)
Comments:  Autophosphorylation on truncated PINK1
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lactacystin increase
CCCP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
Effect of modification (process):  autophagy, induced
Comments:  Parkin recruitment; mitophagy, T313A and S402A mutants interfere with PINK1 phosphorylation; regulate substrate phosphorylation, Parkin and Ubiquitin