Curated Information
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Home > Curated Information Page > PubMed Id: 25512381
Deng SS, et al. (2015) Protein Kinase A Rescues Microtubule Affinity-regulating Kinase 2-induced Microtubule Instability and Neurite Disruption by Phosphorylating Serine 409. J Biol Chem 290, 3149-60 25512381
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S409-p - MARK2 (human)
Modsite: NPKQRRFsDQAAGPA SwissProt Entrez-Gene
Orthologous residues
MARK2 (human): S409‑p, MARK2 iso4 (human): S409‑p, MARK2 iso10 (human): S376‑p, MARK2 iso11 (human): S409‑p, MARK2 iso13 (human): S376‑p, MARK2 iso14 (human): S376‑p, MARK2 (mouse): S409‑p, MARK2 iso3 (mouse): S409‑p, MARK2 (rat): S409‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
H-89 decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited
Effect of modification (process):  cytoskeletal reorganization, neural plasticity
Comments:  1) PKAC inhibits phosphorylation of Tau caused by MARK-2.2) PKAC acts as a negative regulator of MARK-2 in regulating microtubule stability and neurite outgrowth.

S573-p - Tau (rat)
Modsite: NVRSKIGsTENLKHQ SwissProt Entrez-Gene
Orthologous residues
Tau (human): S579‑p, Tau iso2 (human): S204‑p, Tau iso3 (human): S168‑p, Tau iso4 (human): S233‑p, Tau iso5 (human): S262‑p, Tau iso6 (human): S204‑p, Tau iso7 (human): S233‑p, Tau iso8 (human): S262‑p, Tau (mouse): S554‑p, Tau iso3 (mouse): S251‑p, Tau iso7 (mouse): S211‑p, Tau (rat): S573‑p, Tau (cow): S269‑p
Characterization
Methods used to characterize site in vivo western blotting
Relevant cell lines - cell types - tissues:  'neuron, hippocampal'-brain
Cellular systems studied:  primary cultured cells
Species studied:  mouse