Ultanir SK, et al. (2014) MST3 Kinase Phosphorylates TAO1/2 to Enable Myosin Va Function in Promoting Spine Synapse Development. Neuron 84, 968-82 25456499

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T158-p - ARHGAP18 (human) ▲

Modsite: RVEtVSQtLRKKNKQ SwissProt Entrez-Gene Orthologous residues ARHGAP18 (human): T158‑p, ARHGAP18 iso2 (human): T113‑p, ARHGAP18 (mouse): T156‑p, ARHGAP18 (rat): T157‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T202-p - FMNL2 (human) ▲

Modsite: HSALRyNtLPSRRTL SwissProt Entrez-Gene Orthologous residues FMNL2 (human): T202‑p, FMNL2 iso2 (human): ‑, FMNL2 iso3 (human): T202‑p, FMNL2 (mouse): T202‑p, FMNL2 (rat): T202‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T99-p - PAK6 (human) ▲

Modsite: LSVISSNtLRGRsPT SwissProt Entrez-Gene Orthologous residues PAK6 (human): T99‑p, PAK6 (mouse): T99‑p, PAK6 (rat): T99‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T440-p - TAO1 (human) ▲

Modsite: RNREHFAtIRTAsLV SwissProt Entrez-Gene Orthologous residues TAO1 (human): T440‑p, TAO1 iso3 (human): T440‑p, TAO1 (mouse): T440‑p, TAO1 (rat): T440‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MST3 (human) transfection of inactive enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays DBN1 (human) Induces pull-down assay MYL5 (human) Induces intracellular localization pull-down assay, co-immunoprecipitation Comments:  reduced filopodia density, spine development

T468-p - TAO2 (human) ▲
Modsite: RNRDHFAtIRTAsLV SwissProt Entrez-Gene Orthologous residues TAO2 (human): T468‑p, TAO2 iso2 (human): T468‑p, TAO2 (mouse): T475‑p, TAO2 iso2 (mouse): T475‑p, TAO2 (rat): T470‑p, TAO2 iso2 (rat): T476‑p

T156-p - ARHGAP18 (mouse) ▲
Modsite: RVEtVSQtLRKKNKQ SwissProt Entrez-Gene Orthologous residues ARHGAP18 (human): T158‑p, ARHGAP18 iso2 (human): T113‑p, ARHGAP18 (mouse): T156‑p, ARHGAP18 (rat): T157‑p

T20-p - CASP3 (mouse) ▲
Modsite: INNFEVktIHGSksV SwissProt Entrez-Gene Orthologous residues CASP3 (human): I20‑p, CASP3 (mouse): T20‑p, CASP3 (rat): T20‑p

T432-p - EEF1A1 (mouse) ▲
Modsite: AVRDMRQtVAVGVIk SwissProt Entrez-Gene Orthologous residues EEF1A1 (human): T432‑p, EEF1A1 (mouse): T432‑p, EEF1A1 (rat): T432‑p, EEF1A1 (rabbit): T432‑p

T317-p - EPS8 (mouse) ▲

Modsite: GPGEGVLtLRAKPPP SwissProt Entrez-Gene Orthologous residues EPS8 (human): T317‑p, EPS8 (mouse): T317‑p, EPS8 (rat): T317‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T257-p - ERMN (mouse) ▲
Modsite: NSYSRYNtIsYRKIR SwissProt Entrez-Gene Orthologous residues ERMN (human): T260‑p, ERMN (mouse): T257‑p, ERMN (rat): T258‑p

T202-p - FMNL2 (mouse) ▲

Modsite: HSALRYNtLPSRRTL SwissProt Entrez-Gene Orthologous residues FMNL2 (human): T202‑p, FMNL2 iso2 (human): ‑, FMNL2 iso3 (human): T202‑p, FMNL2 (mouse): T202‑p, FMNL2 (rat): T202‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T242-p - GIPC1 (mouse) ▲

Modsite: QLGTGrGtLRLRSRG SwissProt Entrez-Gene Orthologous residues GIPC1 (human): T242‑p, GIPC1 iso2 (human): T145‑p, GIPC1 (mouse): T242‑p, GIPC1 (rat): T242‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T99-p - PAK6 (mouse) ▲

Modsite: LSVISSNtLRGRSPT SwissProt Entrez-Gene Orthologous residues PAK6 (human): T99‑p, PAK6 (mouse): T99‑p, PAK6 (rat): T99‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human

T9-p - SLC7A11 (mouse) ▲
Modsite: VRKPVVAtISKGGYL SwissProt Entrez-Gene Orthologous residues SLC7A11 (human): T9‑p, SLC7A11 (mouse): T9‑p, SLC7A11 (rat): T9‑p

T440-p - TAO1 (mouse) ▲

Modsite: RNREHFAtIRTAsLV SwissProt Entrez-Gene Orthologous residues TAO1 (human): T440‑p, TAO1 iso3 (human): T440‑p, TAO1 (mouse): T440‑p, TAO1 (rat): T440‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MST3 (human) transfection of inactive enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays DBN1 (human) Induces pull-down assay MYL5 (human) Induces intracellular localization pull-down assay, co-immunoprecipitation Comments:  reduced filopodia density, spine development

T475-p - TAO2 (mouse) ▲

Modsite: RNRDHFAtIRTAsLV SwissProt Entrez-Gene Orthologous residues TAO2 (human): T468‑p, TAO2 iso2 (human): T468‑p, TAO2 (mouse): T475‑p, TAO2 iso2 (mouse): T475‑p, TAO2 (rat): T470‑p, TAO2 iso2 (rat): T476‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), neuron-'brain, hippocampus' Cellular systems studied:  cell lines, primary cultured cells Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MST3 (human) transfection of inactive enzyme, siRNA inhibition of enzyme, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays MYL5 (human) Induces intracellular localization pull-down assay, co-immunoprecipitation DBN1 (human) Induces pull-down assay Comments:  reduced filopodia density, spine development

T161-p - TPPP (mouse) ▲
Modsite: tkAVssPtVSRLTDT SwissProt Entrez-Gene Orthologous residues TPPP (human): T162‑p, TPPP (mouse): T161‑p, TPPP (rat): T161‑p, TPPP (cow): T161‑p

T221-p - TSG101 (mouse) ▲
Modsite: VGPSRDGtISEDTIR SwissProt Entrez-Gene Orthologous residues TSG101 (human): T220‑p, TSG101 (mouse): T221‑p, TSG101 (rat): T221‑p