Li N, et al. (2014) Cyclin C is a haploinsufficient tumour suppressor. Nat Cell Biol 16, 1080-91 25344755

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T2511-p - Notch 1 (human) ▲

Modsite: VPEHPFLtPsPEsPD SwissProt Entrez-Gene Orthologous residues Notch 1 (human): T2511‑p, Notch 1 (mouse): T2487‑p, Notch 1 (rat): T2487‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), SF9, T-ALL (T lymphocyte), thymocyte Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK3 (human) KINASE CDK8 (human) KINASE CDK19 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK8 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK19 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK3 (human) phosphopeptide analysis, siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXW7 (human) Induces co-immunoprecipitation

S2513-p - Notch 1 (human) ▲

Modsite: EHPFLtPsPEsPDQW SwissProt Entrez-Gene Orthologous residues Notch 1 (human): S2513‑p, Notch 1 (mouse): S2489‑p, Notch 1 (rat): S2489‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), SF9, T-ALL (T lymphocyte), thymocyte Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK8 (human) KINASE CDK3 (human) KINASE CDK19 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK3 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK19 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK8 (human) phosphopeptide analysis, siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXW7 (human) Induces co-immunoprecipitation

S2516-p - Notch 1 (human) ▲

Modsite: FLtPsPEsPDQWsss SwissProt Entrez-Gene Orthologous residues Notch 1 (human): S2516‑p, Notch 1 (mouse): S2492‑p, Notch 1 (rat): S2492‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  MEF (fibroblast), SF9, T-ALL (T lymphocyte), thymocyte Cellular systems studied:  cell lines, primary cultured cells Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK3 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK19 (human) phosphopeptide analysis, siRNA inhibition of enzyme KINASE CDK8 (human) phosphopeptide analysis, siRNA inhibition of enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation, ubiquitination Effect of modification (process):  carcinogenesis, inhibited Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FBXW7 (human) Induces co-immunoprecipitation