Curated Information
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Home > Curated Information Page > PubMed Id: 25371038
Niu A, Wang B, Li YP (2015) TNF╬▒ shedding in mechanically stressed cardiomyocytes is mediated by Src activation of TACE. J Cell Biochem 116, 559-65 25371038
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T180-p - P38A (rat)
Modsite: RHTDDEMtGyVATRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress increase
siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA
Downstream Regulation
Effect of modification (function):  activity, induced

Y182-p - P38A (rat)
Modsite: TDDEMtGyVATRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress increase
siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA
Downstream Regulation
Effect of modification (function):  activity, induced

Y419-p - Src (rat)
Modsite: RLIEDNEyTARQGAk SwissProt Entrez-Gene
Orthologous residues
Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress increase
siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  signaling pathway regulation

Y702-p - TACE (rat)
Modsite: DKKLDKQyESLSLFH SwissProt Entrez-Gene
Orthologous residues
TACE (human): Y702‑p, TACE (mouse): Y702‑p, TACE (rat): Y702‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  myocyte-heart
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Src (human) inhibition of upstream enzyme, activation of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
mechanical stress increase
PP2 mechanical stress inhibit treatment-induced increase
PP3 mechanical stress inhibit treatment-induced increase
siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA
Downstream Regulation
Effect of modification (function):  activity, induced, phosphorylation
Comments:  p38 is phosphorylated and activated due to TACE phosphorylation.