Niu A, Wang B, Li YP (2015) TNFα shedding in mechanically stressed cardiomyocytes is mediated by Src activation of TACE. J Cell Biochem 116, 559-65 25371038

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T180-p - P38A (rat) ▲

Modsite: RHTDDEMtGyVATRW SwissProt Entrez-Gene Orthologous residues P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA Downstream Regulation Effect of modification (function):  activity, induced

Y182-p - P38A (rat) ▲

Modsite: TDDEMtGyVATRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA Downstream Regulation Effect of modification (function):  activity, induced

Y419-p - Src (rat) ▲

Modsite: RLIEDNEyTARQGAk SwissProt Entrez-Gene Orthologous residues Src (human): Y419‑p, Src iso2 (human): Y425‑p, Src (mouse): Y424‑p, Src iso2 (mouse): Y418‑p, Src (rat): Y419‑p, Src (chicken): Y416‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA Downstream Regulation Effect of modification (function):  activity, induced Effect of modification (process):  signaling pathway regulation

Y702-p - TACE (rat) ▲

Modsite: DKKLDKQyESLSLFH SwissProt Entrez-Gene Orthologous residues TACE (human): Y702‑p, TACE (mouse): Y702‑p, TACE (rat): Y702‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  myocyte-heart Cellular systems studied:  primary cultured cells Species studied:  rat Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Src (human) inhibition of upstream enzyme, activation of upstream enzyme, siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes mechanical stress increase PP2 mechanical stress inhibit treatment-induced increase PP3 mechanical stress inhibit treatment-induced increase siRNA mechanical stress inhibit treatment-induced increase Src-specific siRNA Downstream Regulation Effect of modification (function):  activity, induced, phosphorylation Comments:  p38 is phosphorylated and activated due to TACE phosphorylation.