Curated Information
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Home > Curated Information Page > PubMed Id: 15143187
Argetsinger LS, et al. (2004) Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity. Mol Cell Biol 24, 4955-67 15143187
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y221-p - JAK2 (mouse)
Modsite: VRAKIQDyHILTRKR SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y221‑p, JAK2 (mouse): Y221‑p, JAK2 (rat): Y221‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) phosphopeptide analysis, 2D analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

Y570-p - JAK2 (mouse)
Modsite: VRREVGDyGQLHKTE SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y570‑p, JAK2 (mouse): Y570‑p, JAK2 (rat): Y570‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) phosphopeptide analysis, 2D analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited

Y1007-p - JAK2 (mouse)
Modsite: VLPQDKEyyKVKEPG SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y1007‑p, JAK2 (mouse): Y1007‑p, JAK2 (rat): Y1007‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [JAK2 (mouse)]
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)

Y1008-p - JAK2 (mouse)
Modsite: LPQDKEyyKVKEPGE SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y1008‑p, JAK2 (mouse): Y1008‑p, JAK2 (rat): Y1008‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

Y699-p - STAT5B (rat)
Modsite: TAKAADGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JAK2 (mouse) transfection of wild-type enzyme, transfection of inactive enzyme