Curated Information
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Home > Curated Information Page > PubMed Id: 15121872
Kurzer JH, et al. (2004) Tyrosine 813 is a site of JAK2 autophosphorylation critical for activation of JAK2 by SH2-B beta. Mol Cell Biol 24, 4557-70 15121872
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y785-p - JAK3 (human)
Modsite: NSLIsSDyELLSDPT SwissProt Entrez-Gene
Orthologous residues
JAK3 (human): Y785‑p, JAK3 (mouse): Y781‑p, JAK3 iso2 (mouse): Y1000‑p, JAK3 iso3 (mouse): Y996‑p, JAK3 (rat): Y781‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast), NK 3.3 (natural killer cell)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK3 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IL-2 increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SH2-B-beta (rat) Induces co-immunoprecipitation

Y813-p - JAK2 (mouse)
Modsite: NSLFTPDyELLTEND SwissProt Entrez-Gene
Orthologous residues
JAK2 (human): Y813‑p, JAK2 (mouse): Y813‑p, JAK2 (rat): Y813‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, phosphoamino acid analysis, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout], COS (fibroblast), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  green monkey, human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE JAK2 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
GH increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SH2-B-beta (rat) Induces co-immunoprecipitation

Y699-p - STAT5B (rat)
Modsite: TAKAADGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Comments:  Co-expression with Jak2 and SH2-Bbeta enhances phosphorylation of STAT5B.